Earthworms are an ideal biological model in toxicity assays and environmental monitoring studies. In the present study, the reproductive toxicity and histopathological effects of Monocrotophos pesticide on an exotic epigeic Eudrilus eugeniae and an indigenous epigeic Perionyx barotensis earthworm were studied. Earthworm species were exposed to different concentrations of pesticide like 450 ppm, 500 ppm, and 650 ppm for 45 days and the mortality rate and reproductive activity was recorded every 15 days of exposure. There was an increase in mortality and abnormal sperm (asthenospermia, necrospermia, and oligospermia) and defective cocoons in earthworms with increasing concentrations of the pesticide. Histopathological changes like rupture of chloragogenous tissue, longitudinal muscle, fused and extra-villous growth and necrotic cell rupture in earthworm's body wall (epidermis, circular and longitudinal muscles) were observed. Fluorescent probes have detected cell death in pesticide-treated earthworms when compared to the control group after 45 days. The present findings show that Monocrotophos pesticide on exposure to epigeic earthworm species causes significant reproductive toxicity and histopathological abnormalities and these changes could be used as a tool in environmental risk assessment of pesticides. Abbreviations: DDT: Dichlorodiphenyltrichloroethane; MCP: Monocrotophos; EPA: Environment Protection Act; SL: Soluble Liquid; C: N (Carbon: Nitrogen); C: P (Carbon: Phosphorus); LC: Lethal Concentration; PBS: Phosphate Buffer Solution; WHO: World Health Organization; H&E: Hematoxylin and Eosin; SV: seminal vesicles; O: ovary; GP: genital papillae; Ch: chloragogenous tissue; EL: epithelial layer; CM: circular muscle; LM: longitudinal muscle; CD: cell debris.

中文翻译：

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农业化学农药（Monocrotophos）对本土和外来earth物种的生理和生殖行为的毒性作用研究。

toxicity是毒性测定和环境监测研究中的理想生物学模型。在本研究中，研究了久效磷农药对外来流行的Eudulus eugeniae和本土流行的Perionyx barotensis worm的生殖毒性和组织病理学作用。worm物种暴露于不同浓度的农药（如450 ppm，500 ppm和650 ppm）达45天，每15天记录一次死亡率和生殖活性。随着农药浓度的增加，earth中的死亡率和精子异常（无精子症，坏死性精子症和少精子症）以及有缺陷的茧都有所增加。组织病理学变化，如绿藻组织破裂，纵肌，在earth的体壁（表皮，圆形和纵向肌肉）中观察到融合和绒毛外生长以及坏死细胞破裂。与对照组相比，荧光探针已检测到45天后杀虫earth中的细胞死亡。目前的发现表明久效磷农药暴露于地表earth类动物会引起明显的生殖毒性和组织病理学异常，这些变化可作为评估农药环境风险的一种手段。缩写：DDT：二氯二苯基三氯乙烷；MCP：久效磷；EPA：《环境保护法》；SL：可溶性液体；C：N（碳：氮）；C：P（碳：磷）；LC：致死浓度；PBS：磷酸盐缓冲液；世卫组织：世界卫生组织；H＆E：苏木精和曙红；SV：精囊；O：卵巢；GP：生殖器乳头；Ch：绿藻组织；EL：上皮层；CM：圆肌；LM：纵肌；CD：细胞碎片。