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Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci
Genetica ( IF 1.3 ) Pub Date : 2019-01-22 , DOI: 10.1007/s10709-019-00053-7
C Ribout 1 , A Villers 1, 2 , S Ruault 1 , V Bretagnolle 1, 2 , D Picard 3 , K Monceau 1, 2 , Bertrand Gauffre 1, 2, 4, 5
Affiliation  

The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and contemporary gene flow, we developed 16 new microsatellite markers using 454 pyrosequencing. Genotypes of 117 chicks sampled in a 200 × 300 km farmland area in Central Western France were analyzed to characterize genetic polymorphism at each locus and regional and fine-scale genetic structure. Fourteen markers were found polymorphic, with a number of alleles ranging from 3 to 11. The expected and observed heterozygosities ranged from 0.36 to 0.856 and from 0.35 to 0.868, respectively. A single genetic unit was found at the regional scale with higher genetic similarity observed at a small spatial scale (up to 10 km). Our results are consistent with overall large-scale juvenile and adult dispersal together with small-scale male philopatry. Cross-species amplification of this set of microsatellites makers has been successful in two closely related harrier species: the marsh harrier (Circus aeruginosus) and the Hen harrier (Circus cyaneus) for which 14 and 12 markers were polymorphic, respectively. These new microsatellite markers could be used to study the population genetic structure, contemporary gene flow and parentage analyses in these three species and to conduct microsatellite-based demographic inferences on the Montagu’s harrier.

中文翻译:

一组新的微卫星位点揭示了一种高扩散能力猛禽的精细遗传结构,即蒙塔古鹞 (Circus pygargus)

蒙塔古鹞 (Circus pygargus) 是一种半殖民地猛禽物种,广泛但不规则地分布在整个古北地区,有记录的迁徙案例和额外对交配的存在。为了评估 Montagu 的鹞式空间遗传结构和当代基因流,我们使用 454 焦磷酸测序开发了 16 个新的微卫星标记。分析了在法国中西部 200 × 300 公里农田区域采样的 117 只雏鸡的基因型,以表征每个位点的遗传多态性以及区域和精细尺度的遗传结构。发现 14 个标记是多态的,等位基因的数量范围为 3 到 11。预期和观察到的杂合度分别范围为 0.36 到 0.856 和 0.35 到 0.868。在区域尺度上发现了一个单一的遗传单元,在小空间尺度(最多 10 公里)观察到更高的遗传相似性。我们的结果与整体大规模的青少年和成人散播以及小规模的雄性恋童癖是一致的。这组微卫星标记的跨物种扩增已在两个密切相关的鹞种中取得成功:沼泽鹞 (Circus aeruginosus) 和母鸡 (Circus cyaneus),分别有 14 和 12 个标记是多态的。这些新的微卫星标记可用于研究这三个物种的种群遗传结构、当代基因流和亲本分析,并对蒙塔古鹞进行基于微卫星的人口统计推断。我们的结果与整体大规模的青少年和成人散播以及小规模的雄性恋童癖是一致的。这组微卫星标记的跨物种扩增已在两个密切相关的鹞种中取得成功:沼泽鹞 (Circus aeruginosus) 和母鸡 (Circus cyaneus),分别有 14 和 12 个标记是多态的。这些新的微卫星标记可用于研究这三个物种的种群遗传结构、当代基因流和亲本分析,并对蒙塔古鹞进行基于微卫星的人口统计推断。我们的结果与整体大规模的青少年和成人散播以及小规模的雄性恋童癖是一致的。这组微卫星标记的跨物种扩增已在两个密切相关的鹞种中取得成功:沼泽鹞 (Circus aeruginosus) 和母鸡 (Circus cyaneus),分别有 14 和 12 个标记是多态的。这些新的微卫星标记可用于研究这三个物种的种群遗传结构、当代基因流和亲本分析,并对蒙塔古鹞进行基于微卫星的人口统计推断。沼泽鹞 (Circus aeruginosus) 和母鹞 (Circus cyaneus) 分别有 14 和 12 个标记是多态的。这些新的微卫星标记可用于研究这三个物种的种群遗传结构、当代基因流和亲本分析,并对蒙塔古鹞进行基于微卫星的人口统计推断。沼泽鹞 (Circus aeruginosus) 和母鹞 (Circus cyaneus) 分别有 14 和 12 个标记是多态的。这些新的微卫星标记可用于研究这三个物种的种群遗传结构、当代基因流和亲本分析,并对蒙塔古鹞进行基于微卫星的人口统计推断。
更新日期:2019-01-22
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