The authors aim to report a case of surreptitious administration of a synthetic cannabinoid (SC) and the subsequent toxicological investigations to be able to document accurately the case for submission at a hearing. A dealer gave surreptitiously a substance to two juvenile migrants who experienced shakings and faintness. The laboratory received a blood sample from each of the two victims, who, according to the investigators, were probably exposed to SGT-151, a SC, also known as CUMYL-PEGACLONE. Blood and urine specimens from the dealer, who claimed being a user of SGT-151 were received at the same time. To characterize the metabolites of SGT-151, the drug was incubated with a pool of human hepatic microsomes and the cofactors required to ensure the functioning of the main Phase I and Phase II enzymes. The incubation media were analyzed by liquid chromatography coupled to high-resolution mass spectrometry. The metabolites identified following transformation by hepatic microsomes were mostly N-dealkylated SGT-151, mono-hydroxylated SGT-151 and di-hydroxylated SGT-151. The presence of SGT-151 (5.4 ng/mL) and its metabolite, N-dealkyl SGT-151, was confirmed in the dealer's blood. Two metabolites of SGT-151 (OH-SGT-151, diOH-SGT-151) were detected in the dealer's urine. SGT-151 (~1 ng/mL) and its metabolite N-dealkyl SGT-151 were detected in the blood samples of the two victims.

中文翻译：

---

秘密给药后血液和尿液中SGT-151（CUMYL-PEGACLONE）及其代谢物的测试。

作者的目的是报告一个秘密使用合成大麻素（SC）的病例，随后进行了毒理学调查，以便能够准确记录该病例，以便在听证会上提交。一名商人暗中给两名经历了摇晃和晕眩的少年移民提供了一种物质。实验室从两名受害者的每人那里采集了血液样本，据研究人员说，他们很可能暴露于SGT-151，一种SC，也称为CUMYL-PEGACLONE。同时收到了经销商的血液和尿液标本，他们声称是SGT-151的使用者。为了表征SGT-151的代谢物，将药物与人肝微粒体库和确保主要I期和II期酶功能所需的辅因子一起孵育。通过液相色谱结合高分辨率质谱法分析孵育培养基。肝微粒体转化后鉴定的代谢物主要是N-脱烷基SGT-151，单羟基SGT-151和二羟基SGT-151。在经销商的血液中证实了SGT-151（5.4 ng / mL）及其代谢产物N-脱烷基SGT-151的存在。在经销商的尿液中检测到两种SGT-151代谢产物（OH-SGT-151，diOH-SGT-151）。在两名受害者的血液样本中检测到SGT-151（〜1 ng / mL）及其代谢物N-脱烷基SGT-151。在经销商的血液中确认了4 ng / mL）及其代谢产物N-脱烷基SGT-151。在经销商的尿液中检测到两种SGT-151代谢产物（OH-SGT-151，diOH-SGT-151）。在两名受害者的血液样本中检测到SGT-151（〜1 ng / mL）及其代谢物N-脱烷基SGT-151。在经销商的血液中确认了4 ng / mL）及其代谢产物N-脱烷基SGT-151。在经销商的尿液中检测到两种SGT-151代谢产物（OH-SGT-151，diOH-SGT-151）。在两名受害者的血液样本中检测到SGT-151（〜1 ng / mL）及其代谢物N-脱烷基SGT-151。