ABSTRACT The objective of this study was to clarify the relationship between the effect and associated mechanisms of lithium chloride on neural stem cells (NSCs) and the Wnt signaling pathway. The expression of key molecules proteins related to the Wnt signaling pathway in the proliferation and differentiation of control NSCs and lithium chloride-treated NSCs was detected by Western blot analysis. Flow cytometry analysis was applied to study the cell cycle dynamics of control NSCs and NSCs treated with lithium chloride. The therapeutic concentrations of lithium chloride stimulated NSC proliferation. β-catenin expression gradually decreased, while Gsk-3β expression gradually increased (P < 0.01). Furthermore, NSCs treated with lithium chloride showed significantly enhanced β-catenin expression and inhibited Gsk-3β expression in a dose-dependent manner. NSCs in the G0/G1-phases were activated with an increased therapeutic concentration of lithium chloride, while NSCs in the S-phase, as well as G2/M-phases, were arrested (P < 0.01). These data confirm that the proliferation of NSCs is remarkably promoted through changes of cell dynamics after treatment with lithium chloride. Our results provide insight into the effects of lithium chloride in promoting the proliferation abilities of NSCs in vitro and preventing the cells from differentiating, which is potentially mediated by activation of the Wnt signaling pathway.

中文翻译：

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氯化锂在体外促进神经干细胞增殖，可能是通过触发 Wnt 信号通路

摘要 本研究的目的是阐明氯化锂对神经干细胞 (NSC) 的影响和相关机制与 Wnt 信号通路之间的关系。通过Western印迹分析检测对照NSCs和氯化锂处理的NSCs增殖分化中与Wnt信号通路相关的关键分子蛋白的表达。流式细胞术分析用于研究对照 NSCs 和氯化锂处理的 NSCs 的细胞周期动力学。氯化锂的治疗浓度刺激了 NSC 增殖。β-catenin 表达逐渐降低，而 Gsk-3β 表达逐渐升高（P < 0.01）。此外，用氯化锂处理的 NSCs 显示出显着增强的 β-catenin 表达并以剂量依赖性方式抑制 Gsk-3β 表达。G0/G1 期的神经干细胞随着氯化锂治疗浓度的增加而被激活，而 S 期和 G2/M 期的神经干细胞被抑制（P < 0.01）。这些数据证实，氯化锂处理后细胞动力学的变化显着促进了神经干细胞的增殖。我们的研究结果提供了对氯化锂在体外促进 NSCs 增殖能力和阻止细胞分化的影响的见解，这可能是由 Wnt 信号通路的激活介导的。以及 G2/M 期，被逮捕（P < 0.01）。这些数据证实，氯化锂处理后细胞动力学的变化显着促进了神经干细胞的增殖。我们的研究结果提供了对氯化锂在体外促进 NSCs 增殖能力和阻止细胞分化的影响的见解，这可能是由 Wnt 信号通路的激活介导的。以及 G2/M 期，被逮捕（P < 0.01）。这些数据证实，氯化锂处理后细胞动力学的变化显着促进了神经干细胞的增殖。我们的研究结果提供了对氯化锂在体外促进 NSCs 增殖能力和阻止细胞分化的影响的见解，这可能是由 Wnt 信号通路的激活介导的。