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Effects of hypoxic preconditioning on memory evaluated using the T-maze behavior test
Animal Cells and Systems ( IF 2.5 ) Pub Date : 2019-01-02 , DOI: 10.1080/19768354.2018.1557743
Yun-Hee Kim 1 , Kuen-Su Lee 1 , Young-Sung Kim 2 , Yeon-Hwa Kim 3 , Jae-Hwan Kim 1
Affiliation  

ABSTRACT Perioperative brain ischemia and stroke are leading causes of morbidity and mortality. Brief hypoxic preconditioning is known to have protective effects against hypoxic-ischemic insult in the brain. Current studies on the neuroprotective effects of ischemic preconditioning are based on histologic findings and biomarker changes. However, studies regarding effects on memory are rare. To precondition zebrafish to hypoxia, they were exposed to a dissolved oxygen (DO) concentration of 1.0 ± 0.5 mg/L in water for 30 s. The hypoxic zebrafish were then exposed to 1.0 ± 0.5 mg/L DO until the third stage of hypoxia, for 10 min ± 30 s. Zebrafish were assessed for memory retention after the hypoxic event. Learning and memory were tested using the T-maze, which evaluates memory based on whether or not zebrafish moves to the correct target compartment. In the hypoxic preconditioning group, infarct size was reduced compared with the hypoxic-only treated zebrafish group; memory was maintained to a degree similar to that in the hypoxia-untreated group. The hypoxic-only group showed significant memory impairments. In this study, we used a hypoxic zebrafish model and assessed the effects of ischemic preconditioning not only on histological damages but also on brain function, especially memory. This study demonstrated that a brief hypoxic event has protective effects in hypoxic brain damage and helped maintain memory in zebrafish. In addition, our findings suggest that the zebrafish model is useful in rapidly assessing the effects of ischemic preconditioning on memory.

中文翻译:

使用 T 迷宫行为测试评估缺氧预处理对记忆的影响

摘要 围手术期脑缺血和中风是发病率和死亡率的主要原因。已知短暂的缺氧预处理对大脑中的缺氧缺血性损伤具有保护作用。目前关于缺血预处理的神经保护作用的研究是基于组织学发现和生物标志物的变化。然而,关于对记忆的影响的研究很少。为了使斑马鱼处于缺氧状态,它们在水中暴露于 1.0 ± 0.5 毫克/升的溶解氧 (DO) 浓度 30 秒。然后将缺氧斑马鱼暴露于 1.0 ± 0.5 mg/L DO 直到缺氧的第三阶段,持续 10 分钟 ± 30 秒。在缺氧事件后评估斑马鱼的记忆力。使用 T 迷宫测试学习和记忆,它根据斑马鱼是否移动到正确的目标隔间来评估记忆。在缺氧预处理组中,与仅缺氧处理的斑马鱼组相比,梗塞面积减小;记忆保持程度与缺氧未治疗组相似。仅缺氧组表现出显着的记忆障碍。在这项研究中,我们使用了缺氧斑马鱼模型,并评估了缺血预处理不仅对组织学损伤的影响,而且对大脑功能,尤其是记忆力的影响。这项研究表明,短暂的缺氧事件对缺氧脑损伤具有保护作用,并有助于维持斑马鱼的记忆力。此外,我们的研究结果表明,斑马鱼模型可用于快速评估缺血预处理对记忆的影响。记忆保持程度与缺氧未治疗组相似。仅缺氧组表现出显着的记忆障碍。在这项研究中,我们使用了缺氧斑马鱼模型,并评估了缺血预处理不仅对组织学损伤的影响,而且对大脑功能,尤其是记忆力的影响。这项研究表明,短暂的缺氧事件对缺氧脑损伤具有保护作用,并有助于维持斑马鱼的记忆力。此外,我们的研究结果表明,斑马鱼模型可用于快速评估缺血预处理对记忆的影响。记忆保持程度与缺氧未治疗组相似。仅缺氧组表现出显着的记忆障碍。在这项研究中,我们使用了缺氧斑马鱼模型,并评估了缺血预处理不仅对组织学损伤的影响,而且对大脑功能,尤其是记忆力的影响。这项研究表明,短暂的缺氧事件对缺氧脑损伤具有保护作用,并有助于维持斑马鱼的记忆力。此外,我们的研究结果表明,斑马鱼模型可用于快速评估缺血预处理对记忆的影响。我们使用缺氧斑马鱼模型,评估缺血预处理不仅对组织学损伤的影响,而且对大脑功能,尤其是记忆力的影响。这项研究表明,短暂的缺氧事件对缺氧脑损伤具有保护作用,并有助于维持斑马鱼的记忆力。此外,我们的研究结果表明,斑马鱼模型可用于快速评估缺血预处理对记忆的影响。我们使用缺氧斑马鱼模型,评估缺血预处理不仅对组织学损伤的影响,而且对大脑功能,尤其是记忆力的影响。这项研究表明,短暂的缺氧事件对缺氧脑损伤具有保护作用,并有助于维持斑马鱼的记忆力。此外,我们的研究结果表明,斑马鱼模型可用于快速评估缺血预处理对记忆的影响。
更新日期:2019-01-02
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