The current objective was to evaluate six groups of titanium membranes in a rat calvarial defect model, regarding the surface treatment with or without calcium-phosphate coating and surface topography with no, small, or large holes. Critical size defects (Ф = 8 mm, n = 42) were surgically created in rat calvaria, and then were treated by one of the six groups. Biopsies were obtained at 4 weeks (n = 5 per group) for micro-computed tomography and histomorphometric analyses. Fluorochrome bone markers were injected in two rats each group at 1 (Alizarin red), 3 (Calcein green) and 5 weeks (Oxytetracyclin yellow), followed by histological examination at 7 weeks to assess bone regeneration dynamic. At 4 weeks, the highest bone volume was observed in no-hole groups independent of surface treatment (p < 0.05). Treated groups with no-hole and large-hole membranes showed increased bone mineral density than with respective non-treated groups (p < 0.05). Histology exhibited an intimate bone formation onto the treated membranes, whereas non-treated ones demonstrated interposition of connective tissue, which was confirmed through bone contact percentages. The results suggest that occlusive membranes showed more bone formation than other perforated ones, and calcium-phosphate treatment induces intimate bone formation toward the membrane.

中文翻译：

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通过不同设计的钛膜在有或没有表面处理的情况下进行的体内骨再生：对大鼠颅盖缺损的研究。

目前的目标是在大鼠颅盖膜缺损模型中评估六组钛膜，涉及有无磷酸钙涂层的表面处理以及无，无或大或小孔的表面形貌。在大鼠颅骨中通过外科手术制造了临界尺寸缺陷（Ф= 8 mm，n = 42），然后由六组之一进行治疗。在第4周进行活检（每组n = 5），进行显微计算机断层扫描和组织形态分析。将荧光染料骨标记物分别注射到两只大鼠中，分别在1组（茜素红），3组（钙黄绿素）和5周（土霉素黄）注射，然后在7周进行组织学检查以评估骨再生动态。在第4周，无孔组的最高骨体积与表面处理无关（p <0.05）。与未治疗组相比，无孔和大孔膜治疗组的骨矿物质密度增加（p <0.05）。组织学显示在处理过的膜上有紧密的骨形成，而未处理过的膜显示出结缔组织的插入，这通过骨接触百分数得以证实。结果表明，闭塞膜比其他穿孔膜显示出更多的骨形成，而磷酸钙处理可诱导膜上紧密的骨形成。