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Colono-protective Potentiality of Methanolic Bark Extract of Acacia catechu: A Medicinal Plant against 1,2-Dimethylhydrazine-Induced Toxicity in Wistar Rats.
Journal of Environmental Pathology, Toxicology and Oncology ( IF 2.1 ) Pub Date : 2019-02-27 , DOI: 10.1615/jenvironpatholtoxicoloncol.2018026923
Alpa Shree 1 , Syed Kazim Hasan 1 , Johirul Islam 1 , Abul Vafa 1 , Shekh Mohammad Afzal 1 , Preeti Barnwal 1 , Aisha Siddiqi 1 , Rahat Ali 1 , Sarwat Sultana 1
Affiliation  

The protective efficacy of methanolic bark extract of Acacia catechu Willd. (MEBA) against 1,2-dimethylhydrazine (DMH)-induced colon toxicity was investigated. Acacia catechu is considered one of the most potent medicines for various diseases in Ayurveda, a traditional system of Indian medicine. It is a widely used herb that contains a variety of bioactive components such as phenolic acids, alkaloids, and flavonoids among others. In the present study, MEBA was used as a pretreatment orally at two doses (250 and 500 mg/kg body weight [b.w.] once daily for 7 days), and DMH was administered (at a dose of 40 mg/kg b.w.) subcutaneously on day 7 in Wistar rats. The protective potential of MEBA was assessed in terms of the activity of antioxidant enzymes, lipid peroxidation, and expression of inflammatory markers (iNOS, COX-2, NF-κB, IL-6). Pretreatment with MEBA significantly abrogated oxidative damage by diminishing tissue lipid peroxidation, increasing enzymatic activities of various antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione), and diminishing the induced expression of inflammatory markers in the colon tissue of Wistar rats. Furthermore, histopathological findings revealed that pretreatment with (MEBA) reduced intense filtration of inflammatory cells and significantly restored the architecture of colonic tissue. The results of this study indicate that MEBA significantly suppresses DMH-induced toxicity by ameliorating oxidative stress and inflammation and by restoring the architecture of colon tissue.

中文翻译:

刺槐相思甲醇树皮提取物的结肠保护潜力:一种抗1,2-二甲基肼诱导的Wistar大鼠毒性的药用植物。

刺槐金合欢树皮提取物的保护作用。(MEBA)对1,2-二甲基肼(DMH)诱导的结肠毒性进行了研究。相思金合欢被认为是印度传统医学系统阿育吠陀中对各种疾病最有效的药物之一。它是一种广泛使用的草药,其中包含多种生物活性成分,例如酚酸,生物碱和类黄酮。在本研究中,MEBA用作口服预处理剂,剂量为两次(每天250和500 mg / kg体重[bw],连续7天,每天一次),并皮下注射DMH(剂量为40 mg / kg bw)在第7天出现Wistar大鼠。根据抗氧化酶的活性,脂质过氧化和炎症标志物(iNOS,COX-2,NF-κB,IL-6)的表达评估了MEBA的保护潜力。用MEBA预处理可通过减少组织脂质过氧化作用,增加各种抗氧化酶(过氧化氢酶,谷胱甘肽过氧化物酶,谷胱甘肽还原酶,谷胱甘肽S-转移酶,减少谷胱甘肽)的酶活性,并减少结肠中炎性标志物的诱导表达来消除氧化损伤。 Wistar大鼠的组织。此外,组织病理学结果显示,用(MEBA)进行的预处理可减少炎症细胞的强烈过滤并显着恢复结肠组织的结构。这项研究的结果表明,MEBA可通过减轻氧化应激和炎症以及恢复结肠组织的结构来显着抑制DMH诱导的毒性。增加各种抗氧化酶(过氧化氢酶,谷胱甘肽过氧化物酶,谷胱甘肽还原酶,谷胱甘肽S-转移酶,还原型谷胱甘肽)的酶活性,并减少Wistar大鼠结肠组织中炎症标记物的诱导表达。此外,组织病理学结果显示,用(MEBA)预处理可减少炎症细胞的强烈过滤并显着恢复结肠组织的结构。这项研究的结果表明,MEBA可通过减轻氧化应激和炎症以及恢复结肠组织的结构来显着抑制DMH诱导的毒性。增加各种抗氧化酶(过氧化氢酶,谷胱甘肽过氧化物酶,谷胱甘肽还原酶,谷胱甘肽S-转移酶,还原型谷胱甘肽)的酶活性,并减少Wistar大鼠结肠组织中炎症标记物的诱导表达。此外,组织病理学结果显示,用(MEBA)进行的预处理可减少炎症细胞的强烈过滤并显着恢复结肠组织的结构。这项研究的结果表明,MEBA可通过减轻氧化应激和炎症以及恢复结肠组织的结构来显着抑制DMH诱导的毒性。并减少Wistar大鼠结肠组织中炎症标志物的诱导表达。此外,组织病理学结果显示,用(MEBA)进行的预处理可减少炎症细胞的强烈过滤并显着恢复结肠组织的结构。这项研究的结果表明,MEBA可通过减轻氧化应激和炎症以及恢复结肠组织的结构来显着抑制DMH诱导的毒性。并减少Wistar大鼠结肠组织中炎症标志物的诱导表达。此外,组织病理学结果显示,用(MEBA)进行的预处理可减少炎症细胞的强烈过滤并显着恢复结肠组织的结构。这项研究的结果表明,MEBA可通过减轻氧化应激和炎症以及恢复结肠组织的结构来显着抑制DMH诱导的毒性。
更新日期:2019-11-01
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