BackgroundBladder cancer was a malignant disease in patients, our research aimed at discovering the possible biomarkers for the diseases.ResultsThe gene chip GSE31684, including 93samples, was downloaded from the GEO datasets and co-expression network was constructed by the data. Molecular complex detection(MCODE) was used to identify hub genes. The most significant cluster including 16 genes: CDH11, COL3A1, COL6A3, COL5A1, AEBP1, COL1A2, NTM, COL11A1, THBS2, COL8A1, COL1A1, BGN, MMP2, PXDN, THY1, and TGFB1I1 was identified. After annotated by BiNGO, they were suggested associated with collagen fibril organization and blood vessel development. In addition, the Kaplan Meier curves were obtained by UALCAN. The high expression of THY1, AEBP1, CDH11, COL1A1, COL1A2, COL11A1, MMP2, PXDN, BGN, COL5A1, COL8A1, and TGFB1I1 indicated poor prognosis of the patients(P < 0.05). Finally, we examined genes’ expression between low and high tumor stage by the Wilcoxon test(P < 0.05), TGFB1I1 was excluded.ConclusionTHY1, AEBP1, CDH11, COL1A1, COL1A2, COL11A1, MMP2, PXDN, BGN, COL5A1, COL8A1 associated with the tumor stage as well as tumor patients’ prognosis. COL5A1, COL8A1(P < 0.01) may serve as therapeutic targets for the disease.

中文翻译：

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WGCNA 共表达网络分析揭示了膀胱癌分期相关的中枢基因

背景膀胱癌是患者的恶性疾病，我们的研究旨在发现该疾病可能的生物标志物。结果从GEO数据集中下载基因芯片GSE31684，包括93个样本，并利用数据构建共表达网络。分子复合物检测（MCODE）用于鉴定中心基因。最重要的簇包括 16 个基因：CDH11、COL3A1、COL6A3、COL5A1、AEBP1、COL1A2、NTM、COL11A1、THBS2、COL8A1、COL1A1、BGN、MMP2、PXDN、THY1 和 TGFB1I1。经 BiNGO 注释后，它们被认为与胶原纤维组织和血管发育有关。此外，Kaplan Meier 曲线由 UALCAN 获得。THY1、AEBP1、CDH11、COL1A1、COL1A2、COL11A1、MMP2、PXDN、BGN、COL5A1、COL8A1、TGFB1I1提示患者预后不良(P < 0.05)。最后，我们通过Wilcoxon检验检测了肿瘤低分期和高分期之间的基因表达情况（P < 0.05），排除了TGFB1I1。结论THY1，AEBP1，CDH11，COL1A1，COL1A2，COL11A1，MMP2，PXDN，BGN，COL5A1，COL8A1与肿瘤分期以及肿瘤患者的预后。COL5A1、COL8A1(P < 0.01)可作为该疾病的治疗靶点。