BackgroundTo determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the risk of lead poisoning.ResultsSerum AGT levels were significantly higher in individuals carrying the rs7079 CA genotype, as compared to those carrying the rs7079 CC genotype. The binding of the miRNA mimics miR-31-5p and miR-584-5p to the 3′ UTR of AGT differed based on which rs7079 variant was present, implying that AGT gene expression depends on the rs7079 variant carried.ConclusionsThe rs7079 C to A substitution reduced the binding of miR-31-5p/miR-584-5p to the 3′ UTR of AGT, possibly altering the risk of lead poisoning.

中文翻译：

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铅中毒机制新模型：miRNA靶区SNP影响AGT表达水平

背景确定位于血管紧张素原基因（AGT）3'UTR的rs7079多态性是否改变了AGT基因的表达和铅中毒的风险。一项病例对照研究和荧光素酶报告基因检测确定了 rs7079 变异体与铅中毒风险之间的显着关联。结果与携带 rs7079 CC 基因型的个体相比，携带 rs7079 CA 基因型的个体的血清 AGT 水平显着更高。miRNA 模拟 miR-31-5p 和 miR-584-5p 与 AGT 的 3'UTR 的结合因存在 rs7079 变体而异，这意味着 AGT 基因表达取决于携带的 rs7079 变体。结论 rs7079 C 到 A替代降低了 miR-31-5p/miR-584-5p 与 AGT 的 3'UTR 的结合，可能会改变铅中毒的风险。