Although there are thousands of formalin-fixed paraffin-embedded (FFPE) tissue blocks potentially available for scientific research, many are of questionable quality, partly due to unknown preanalytical variables. We analyzed FFPE tissue biospecimens as part of the National Cancer Institute (NCI) Biospecimen Preanalytical Variables program to identify mRNA markers denoting cold ischemic time. The mRNA was extracted from colon, kidney, and ovary cancer FFPE blocks (40 patients, 10-12 hr fixation time) with 1, 2, 3, and 12 hr cold ischemic times, then analyzed using qRT-PCR for 23 genes selected following a literature search. No genes tested could determine short ischemic times (1-3 hr). However, a combination of three unstable genes normalized to a more stable gene could generate a "Cold Ischemia Score" that could distinguish 1 to 3 hr cold ischemia from 12 hr cold ischemia with 62% sensitivity and 84% specificity.

中文翻译：

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寒冷缺血评分：在福尔马林固定，石蜡包埋的组织中检测到扩展的寒冷缺血的一种mRNA分析。

尽管有成千上万的福尔马林固定石蜡包埋（FFPE）组织块可用于科学研究，但许多块块的质量值得怀疑，部分原因是前分析变量未知。我们分析了FFPE组织生物标本，作为美国国家癌症研究所（NCI）生物标本分析前计划的一部分，以鉴定表示冷缺血时间的mRNA标志。从结肠，肾脏和卵巢癌FFPE块（40例患者，固定时间10-12小时）中提取冷，缺血时间分别为1、2、3和12小时的mRNA，然后使用qRT-PCR分析以下23种基因文献搜索。没有基因测试可以确定短暂的缺血时间（1-3小时）。但是，将三个不稳定基因标准化为更稳定的基因的组合可能会产生“冷缺血评分”