OBJECTIVE The aim of this study was to determine which factors lead to the susceptibility to mouse cytomegalovirus (MCMV) in the spleens of BALB/c mice. METHODS BALB/c and C57BL/6 mice were randomly divided into a control group and an infection group and sacrificed on day 0, 1, 3, 7, 14, and 28 postinfection. The cytotoxicity of NK cells was determined by evaluating lactate dehydrogenase contents. Flow cytometry was used to analyze activated NK cells, IFNγ+ NK cells, and total NK cells in the spleen. The pathological changes of spleens in each group were analyzed by HE staining. The expression of IL10, IL18, IFNγ, Thpok, and IFNβ of spleens was determined by quantitative reverse transcriptase PCR. The viral loads of MCMV in spleens and salivary glands were also detected. RESULTS We found that spleen NK cells and IL10 in C57BL/6 mice possessed more powerful immunity to MCMV than BALB/c mice. In BALB/c mice, combined effects of the cytotoxicity of NK cells and IFNγ in spleens still ended up with deficient control of infection. CONCLUSION The functional shortage of NK cells and inappropriate expression of IL10 result in the susceptibility to MCMV in BALB/c mice.

中文翻译：

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IL10和NK细胞对BALB / c小鼠对小鼠巨细胞病毒敏感性的影响，尽管有IFNγ的补偿。

目的本研究的目的是确定哪些因素导致BALB / c小鼠脾脏对小鼠巨细胞病毒（MCMV）的易感性。方法将BALB / c和C57BL / 6小鼠随机分为对照组和感染组，并在感染后第0、1、3、7、14和28天处死。通过评估乳酸脱氢酶含量来确定NK细胞的细胞毒性。流式细胞仪用于分析脾脏中活化的NK细胞，IFNγ+ NK细胞和总NK细胞。HE染色观察各组脾脏病理变化。通过定量逆转录酶PCR确定脾脏中IL10，IL18，IFNγ，Thpok和IFNβ的表达。还检测了脾和唾液腺中MCMV的病毒载量。结果我们发现，C57BL / 6小鼠的脾脏NK细胞和IL10比BALB / c小鼠具有更强的MCMV免疫力。在BALB / c小鼠中，脾脏中NK细胞和IFNγ的细胞毒性的联合作用仍然导致感染控制不足。结论NK细胞功能不足和IL10表达不当导致BALB / c小鼠对MCMV易感。