We investigated the effects of silver nanoparticle (AgNP) exposure in three ovarian cancer cell lines (A2780, SKOV3, and OVCAR3). We found that AgNPs were highly cytotoxic toward A2780 and SKOV3 cells but OVCAR3 cells were less sensitive to AgNPs. In agreement with the cytotoxicity data, AgNPs caused DNA damage in A2780 and SKOV3 cells, but not in OVCAR3 cells. A2780 and SKOV3 showed higher levels of basal reactive oxygen species (ROS) relative to OVCAR3 cells. AgNP exposure increased ROS levels in both A2780 and SKOV3 cells, but not in OVCAR3 cells. We found that the heterogeneous cytotoxicity was specific to the uptake of intact particles and was not due to differences in sensitivity to silver ions. Furthermore, the combination of AgNPs and standard-of-care platinum therapy, cisplatin (cis-diamminedichloroplatinum(II), CDDP), was synergistic for treatment of A2780 and OVCAR3 cells and the combination of AgNPs and CDDP showed a favorable dose reduction in all cell lines tested. These results provide insight into potential applications of AgNPs for treatment of ovarian cancer.

中文翻译：

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卵巢癌细胞对银纳米颗粒的单一反应以及与顺铂结合的异质性反应。

我们研究了银纳米颗粒（AgNP）暴露在三种卵巢癌细胞系（A2780，SKOV3和OVCAR3）中的作用。我们发现AgNP对A2780和SKOV3细胞具有高度的细胞毒性，但是OVCAR3细胞对AgNP的敏感性较低。与细胞毒性数据一致，AgNP在A2780和SKOV3细胞中引起DNA损伤，但在OVCAR3细胞中没有引起DNA损伤。相对于OVCAR3细胞，A2780和SKOV3显示较高水平的基础活性氧（ROS）。AgNP暴露会增加A2780和SKOV3细胞中的ROS水平，但不会增加OVCAR3细胞中的ROS水平。我们发现异质细胞毒性是对完整颗粒摄取的特异性，而不是由于对银离子敏感性的差异。此外，AgNP与护理标准铂疗法顺铂（cis-diamminedichloroplatinum（II）（CDDP）与A2780和OVCAR3细胞协同治疗，AgNPs和CDDP的组合在所有测试的细胞系中均显示出良好的剂量降低。这些结果为了解AgNPs在卵巢癌治疗中的潜在应用提供了见识。