当前位置: X-MOL 学术Islets › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata.
Islets ( IF 1.9 ) Pub Date : 2018-02-02 , DOI: 10.1080/19382014.2017.1417716
Bashar Khiatah 1 , Amber Tucker 1 , Kuan-Tsen Chen 1 , Rachel Perez 1 , Shiela Bilbao 1 , Luis Valiente 1 , Leonard Medrano 1 , Jeffrey Rawson 1 , Elena Forouhar 1 , Keiko Omori 1 , Fouad Kandeel 1 , Meirigeng Qi 1 , Ismail H Al-Abdullah 1
Affiliation  

Selection of enzymes for optimal pancreas digestion is essential for successful human islet isolations. The aim of this study was to evaluate the efficacy and outcome of using Collagenase Gold plus BP protease (VitaCyte) (n = 8) by comparing it to two commercially available enzymes, Liberase MTF C/T (Roche) (n = 48) and Collagenase NB1/NP (Serva) (n = 15). The isolation outcomes were assessed by islet counting, viability, glucose-stimulated oxygen consumption rate (OCR), and successful graft-rate following transplantation in diabetic NOD scid mice. The pancreas donor characteristics were not significantly different between the tested enzyme groups regarding their BMI, pancreas weight, cold ischemia time (CIT) and HbA1c. The results show that digested tissue volume was not statistically significant between the VitaCyte enzyme (34.25 ± 5.4 mL) and the Roche enzyme (55.25 ± 3.42 mL, p = 0.073), however, this was significant with Serva enzyme (64.07 ± 7.95 mL, p = 0.020). Interestingly, the islet yields were not statistically different between all enzyme groups. Moreover, when islets were transplanted into NOD scid mice, the reversal rate of diabetes for the VitaCyte enzyme group was similar to all enzyme groups. In conclusion, the effectiveness of Collagenase Gold plus BP protease is comparable to the MTF C/T and the Collagenase NB1/NP enzymes; the low cost could facilitate the use of more pancreata for islet isolations.



中文翻译:

胶原酶金加BP蛋白酶在分离人胰岛中的评估。

选择酶以优化胰腺消化对于成功分离人胰岛至关重要。这项研究的目的是通过将胶原酶金加BP蛋白酶(VitaCyte)(n = 8)与两种市售酶Liberase MTF C / T(Roche)(n = 48)和胶原酶NB1 / NP(Serva)(n = 15)。通过胰岛计数,生存力,葡萄糖刺激的耗氧率(OCR)和在糖尿病NOD scid小鼠中移植后的成功移植率来评估分离结果。在测试的酶组之间,其BMI,胰腺重量,冷缺血时间(CIT)和HbA1c的胰腺供体特征无显着差异。结果显示,VitaCyte酶之间的消化组织体积无统计学意义(34.25±5)。4 mL)和Roche酶(55.25±3.42 mL,p = 0.073),但是,对于Serva酶(64.07±7.95 mL,p = 0.020)有意义。有趣的是,所有酶组之间的胰岛产量均无统计学差异。此外,当将胰岛移植到NOD scid小鼠中时,VitaCyte酶组的糖尿病逆转率与所有酶组相似。总之,胶原酶金加BP蛋白酶的功效与MTF C / T和胶原酶NB1 / NP酶相当。低成本可以促进更多胰岛用于胰岛分离。当将胰岛移植到NOD scid小鼠中时,VitaCyte酶组的糖尿病逆转率与所有酶组相似。总之,胶原酶金加BP蛋白酶的功效与MTF C / T和胶原酶NB1 / NP酶相当。低成本可以促进更多胰岛用于胰岛分离。当将胰岛移植到NOD scid小鼠中时,VitaCyte酶组的糖尿病逆转率与所有酶组相似。总之,胶原酶金加BP蛋白酶的功效与MTF C / T和胶原酶NB1 / NP酶相当。低成本可以促进更多胰岛用于胰岛分离。

更新日期:2018-02-02
down
wechat
bug