The centric diatom Cyclotella cryptica and two strains of the pennate diatom Phaeodactylum tricornutum were grown under low and high light intensities (300 lux and 3,000 lux) over 4–6 weeks. Growth was monitored by repetitive cell count. The culture media were replaced weekly to avoid morphological and biochemical alterations caused by nutrient depletion. The ultrastructure of the cells was examined by transmission electron microscopy. Alterations in the light-harvesting antenna systems were investigated by Western immunoblotting. Both diatoms reduced the plastid area, i.e. decreased the amount of thylakoid lamellae, under high light intensity. The thylakoids still ran in groups of three with parallel orientation within the chloroplasts. The girdle band lamellae were not affected. The amounts of storage compounds and vacuoles increased. SDS-PAGE of total cell protein followed by Western immunoblotting with antisera directed against subunits of the light-harvesting antenna systems of C. cryptica (cc-antiserum) and the cryptophyte Cryptomonas maculata (cmac-antiserum) revealed that both diatoms reduced the amount of antenna polypeptides under increased light intensity. The cc-antiserum immunodecorated two bands with relative molecular masses (M _r) of 18,000 and 22,000 in C. cryptica. Both decreased under high light conditions to 67.2±6.1%. Five to seven bands in the M _r range of 14,000–27,000 were recognized in P. tricornutum. They decreased to 83±5.3%. Furthermore, the immunolabeling pattern for both strains differed under the two light regimes. The cmac-antiserum immunodecorated two polypeptides with M _r of 24,000 and 23,000 in C. cryptica, while both strains of P. tricornutum had five polypeptides in the M _r range of 14,000–24,000 that showed some differences in staining intensities between the two strains and in response to the light intensity applied.

中文翻译：

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硅藻的光合作用装置响应于低光强度和高光强度的变化。

中心硅藻Cyclotella cryptica和两株剑状硅藻Phaeodactylum tricornutum在低光照和高光照强度（300 lux和3,000 lux）下生长4-6周。通过重复细胞计数监测生长。每周更换一次培养基，以避免营养耗尽引起的形态和生化变化。通过透射电子显微镜检查细胞的超微结构。通过Western免疫印迹研究了光收集天线系统的变化。在高光强度下，两个硅藻都减少了质体面积，即减少了类囊体薄片的数量。类囊体仍在叶绿体中以三个方向平行排列运行。腰带薄片不受影响。储存化合物和液泡的数量增加。C. cryptica（cc-antiserum）和隐藻Cryptomonas maculata（cmac-antiserum）揭示，在增加的光强度下，两个硅藻都减少了天线多肽的数量。cc-抗坏血酸免疫修饰了隐孢子虫中相对分子质量（M _r）为18,000和22,000的两个条带。两者在强光条件下均降低至67.2±6.1％。在五到七阶中号 _[R的14,000-27,000范围内被认可三角褐指藻。他们下降到83±5.3％。此外，在两种光照方案下，两种菌株的免疫标记模式均不同。cmac-抗死皮瘤用M _r免疫修饰两个多肽的24000和23000中C.隐秘，而两种菌株三角褐指藻曾在5种多肽中号 _ř的14,000-24,000范围的显示出染色的两个菌株之间，并且响应于所施加的光强度的强度有所不同。