Diabetic nephropathy (DN) is a microvascular complication that affects up to 40% of diabetic patients and can lead to end-stage kidney disease. Inflammatory cytokines such as interleukin 1 (IL-1), IL-6, IL-18 and tumor necrosis factor-α (TNFα) have been linked to the development and progression of DN. The aim of our study was to examine the relationship between interleukin-4 (IL4) -590C/T (rs2243250) gene polymorphism and DN in patients with type 2 diabetes mellitus (T2DM). This study is a continuation of our previous research on the association between angiotensinogen (AGT) gene polymorphisms and DN in patients with T2DM. We included 651 unrelated Slovenian (Caucasian) patients who had had T2DM for at least 10 years. The participants were classified into a group of T2DM patients with DN (276 cases) and a group without DN (375 controls). IL4 rs2243250 polymorphism was analyzed using a TaqMan SNP genotyping assay and StepOne Real-Time PCR System. The frequencies of rs2243250 TT, CT and CC (wild type) genotypes were 3.2%, 29.4% and 67.4%, respectively in patients with DN, and 2.7%, 34.4% and 62.9%, respectively in controls. Our logistic regression analysis adjusted for gender, age, diabetes duration, and glycated hemoglobin showed no association between rs2243250 and the risk for DN (OR 1.06; CI 0.37-3.05; p = 0.9). IL4 rs2243250 is not associated with DN in our subset of Slovenian patients with T2DM.

中文翻译：

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在2型糖尿病（T2DM）的白种人中，白介素4（IL4）-590C / T（rs2243250）基因多态性与糖尿病性肾病（DN）不相关。

糖尿病肾病（DN）是一种微血管并发症，可影响多达40％的糖尿病患者，并可导致终末期肾脏疾病。炎症细胞因子如白介素1（IL-1），IL-6，IL-18和肿瘤坏死因子-α（TNFα）与DN的发生和发展有关。我们研究的目的是检查2型糖尿病（T2DM）患者的白细胞介素4（IL4）-590C / T（rs2243250）基因多态性与DN之间的关系。这项研究是我们先前关于T2DM患者血管紧张素原（AGT）基因多态性与DN之间关系的研究的延续。我们纳入了651例不相关的斯洛文尼亚（高加索）患者，他们患有T2DM至少10年。参与者分为DN的T2DM患者组（276例）和无DN的组（375例对照）。使用TaqMan SNP基因分型分析和StepOne实时PCR系统分析IL4 rs2243250多态性。DN患者中rs2243250 TT，CT和CC（野生型）基因型的频率分别为3.2％，29.4％和67.4％，在对照组中分别为2.7％，34.4％和62.9％。我们的逻辑回归分析针对性别，年龄，糖尿病病程和糖化血红蛋白进行了调整，显示rs2243250与DN风险之间没有关联（OR 1.06； CI 0.37-3.05； p = 0.9）。在我们的斯洛文尼亚T2DM患者子集中，IL4 rs2243250与DN不相关。我们的逻辑回归分析针对性别，年龄，糖尿病病程和糖化血红蛋白进行了调整，显示rs2243250与DN风险之间没有关联（OR 1.06； CI 0.37-3.05； p = 0.9）。在我们的斯洛文尼亚T2DM患者子集中，IL4 rs2243250与DN不相关。我们的逻辑回归分析针对性别，年龄，糖尿病病程和糖化血红蛋白进行了调整，显示rs2243250与DN风险之间没有关联（OR 1.06； CI 0.37-3.05； p = 0.9）。在我们的斯洛文尼亚T2DM患者子集中，IL4 rs2243250与DN不相关。