Biogas production from animal waste is an economically viable way to reduce environmental pollution and produce valuable products, i.e., methane and a nutrient-rich organic waste product. An anaerobic digestion reactor for biogas production from pig waste was sampled at the entrance, middle (digestion chamber), and exit of a digester, while the bacterial and archaeal community structure was studied by 16S rRNA gene metagenomics. The number of bacterial operational taxonomic units (OTU)-97% was 3-7 times larger than that of archaeal ones. Bacteria and Archaea found in feces of animals (e.g., Clostridiaceae, Lachnospiraceae, Ruminococcaceae, Methanosarcina, Methanolobus, Methanosaeta, and Methanospirillum) dominated the entrance of the digester. The digestion chamber was dominated by anaerobic sugar-fermenting OP9 bacteria and the syntrophic bacteria Candidatus Cloacamonas (Waste Water of Evry 1; WWE1). The methanogens dominant in the digestion chamber were the acetoclastic Methanosaeta and the hydrogenothrophic Methanoculleus and Methanospirillum. Similar bacterial and archaeal groups that dominated in the middle of the digestion chamber were found in the waste that left the digester. Predicted functions associated with degradation of xenobiotic compounds were significantly different between the sampling locations. The microbial community found in an anaerobic digestion reactor loaded with pig manure contained microorganisms with biochemical capacities related to the 4 phases of methane production.

中文翻译：

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厌氧消化反应器（泻湖型）中的古细菌和细菌群落结构，用于猪场的沼气生产。

用动物粪便生产沼气是减少环境污染并生产有价值的产品（例如甲烷和营养丰富的有机废物）的经济可行的方法。在消化池的入口，中部（消化室）和出口处取样了一个用于从猪粪中产生沼气的厌氧消化反应器，同时通过16S rRNA基因宏基因组学研究了细菌和古细菌的群落结构。细菌操作分类单位（OTU）的数量为-97％，是古细菌的3-7倍。在动物粪便中发现的细菌和古细菌占主导地位的是消化池的入口，该动物的粪便例如梭菌科，鞭毛纲科，瘤胃菌科，甲烷菌属，甲醇客体，甲烷菌属和甲烷螺旋菌。消化室主要由厌氧发酵糖的OP9细菌和营养细菌Candidatus Cloacamonas（Evry 1的废水； WWE1）主导。在消化室中占主导地位的产甲烷菌是乙酰碎屑性甲烷菌和氢致营养性甲烷菌和甲烷螺旋菌。在离开消化池的废物中发现了类似的细菌和古细菌群，这些细菌和消化菌在消化室的中部占主导地位。与异种化合物降解相关的预测功能在采样位置之间存在显着差异。在装有猪粪的厌氧消化反应器中发现的微生物群落包含的微生物具有与甲烷生产的四个阶段有关的生化能力。在消化室中占主导地位的产甲烷菌是乙酰碎屑性甲烷菌和氢致营养性甲烷菌和甲烷螺旋菌。在离开消化池的废物中发现了类似的细菌和古细菌群，这些细菌和消化菌在消化室的中部占主导地位。与异种化合物降解相关的预测功能在采样位置之间存在显着差异。在装有猪粪的厌氧消化反应器中发现的微生物群落包含的微生物具有与甲烷生产的四个阶段有关的生化能力。在消化室中占主导地位的产甲烷菌是乙酰碎屑性甲烷菌和氢致营养性甲烷菌和甲烷螺旋菌。在离开消化池的废物中发现了类似的细菌和古细菌群，这些细菌和消化菌在消化室的中部占主导地位。与异种化合物降解相关的预测功能在采样位置之间存在显着差异。在装有猪粪的厌氧消化反应器中发现的微生物群落包含的微生物具有与甲烷生产的四个阶段有关的生化能力。与异种化合物降解相关的预测功能在采样位置之间存在显着差异。在装有猪粪的厌氧消化反应器中发现的微生物群落包含的微生物具有与甲烷生产的四个阶段有关的生化能力。与异种化合物降解相关的预测功能在采样位置之间存在显着差异。在装有猪粪的厌氧消化反应器中发现的微生物群落包含的微生物具有与甲烷生产的四个阶段有关的生化能力。