Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.

中文翻译：

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用作农杆菌Cohnella sp。生产内切β-木聚糖酶的原料的农业添加剂。应变AR92。

Cohnella sp。的成员。从多种环境中分离出的糖苷水解酶已被证明是生产者。但是，大多数对该属成员的评估仅限于其分类学描述。先前鉴定为属于Cohnella属的AR92菌株与C. thailandensis和C. formosensis（> 80％自举置信度）形成了一个良好支持的簇。它的生长和木聚糖酶的生产是通过使用一种以碱预处理的甘蔗渣为主要碳源的矿物基培养基来进行的，据测定该培养基是生产可能适合其降解的生物催化剂的便捷来源。通过两步统计方法，木聚糖内切酶的生产得到了适度的提高（20％）。但是，观察到的改进要大得多（145％），通过增加接种量并将发酵温度降低到25°C，这低于菌株AR92的最佳生长温度（37°C）。Cohnella sp。生产的木聚糖分解制剂。AR92的主要活性包含温和的温度活性内切木聚糖酶（鉴定为多余的GH10家族），从而导致桦木木聚糖的主要产物木糖和木糖寡糖。