Gametophytes ofOsmunda regalis were culturedin vitro to determine the optimal conditions for growth and development. Culture media with low ammonium concentration (Knop, Knudson or 1/4 Murashige and Skoog dilution) were the most effective for growth of this organism. Addition of sucrose and mannitol to the culture medium at the same osmoticum, inhibits or enhances, respectively, growth and development of the gametophytes, which show a strong autotrophyin vitro. In darkness, both two-dimensional growth and sexual organ formation took place. Gemmae formation is described for the first time in this species and this process does not occur without sucrose in the culture medium or in darkness.

中文翻译：

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养殖的Osmunda regalis配子体中的发芽。

在体外培养Osmunda regalis的配子体以确定最佳的生长发育条件。铵浓度低的培养基（Knop，Knudson或1/4 Murashige和Skoog稀释液）对这种生物的生长最有效。在同一渗透压下向培养基中添加蔗糖和甘露醇分别抑制或增强配子体的生长和发育，其在体外表现出强的自养作用。在黑暗中，二维生长和性器官形成都发生了。在该物种中首次描述了芽孢杆菌的形成，如果在培养基中或在黑暗中没有蔗糖，则不会发生该过程。