Somatic embryos ofGnetum ula (Gnetum edule) an endangered gymnosperm closely related to the angiosperms have been induced in vitro. Megagametophyte tissue with immature embryos was cultured on Murashige and Skoog medium. A mucilaginous, translucent embryogenic callus was obtained with 5 mg/l BA. Callus induced with 2,4-D was non-embryogenic. The embryogenic callus in liquid half strength Murashige and Skoog medium without inorganic nitrates supplemented with 2.5 g/l casein hydrolysate and 0.5 g/l L-glutamine gave rise to immature embryos. The embryos matured when treated with 60 g/l sucrose and 10 mg/l abscisic acid.

中文翻译：

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Gnetum ula Brongn中的体细胞胚胎发生。（Gnetum edule）（Willd）Blume。

已经在体外诱导了与被子植物密切相关的濒危裸子植物Gnetum ula（Gnetum edule）的体细胞胚。在Murashige和Skoog培养基上培养具有未成熟胚的大型配子体组织。用5 mg / l BA获得粘液状，半透明的胚性愈伤组织。2,4-D诱导的愈伤组织是非胚性的。在不添加无机硝酸盐，添加2.5 g / l酪蛋白水解产物和0.5 g / l L-谷氨酰胺的液态半强度Murashige和Skoog培养基中，胚性愈伤组织产生了未成熟的胚。当用60 g / l蔗糖和10 mg / l脱落酸处理时，胚成熟。