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Enhanced production of scopolin bySolanum aviculare cells immobilised within Ca-alginate gel beads.
Plant Cell Reports ( IF 5.3 ) Pub Date : 1997-02-01 , DOI: 10.1007/bf01088295
C Roisin 1 , F Gillet-Manceau 2 , J E Nava Saucedo 1 , M Fliniaux 2 , A Jacquin-Dubreuil 2 , J -N Barbotin 1
Affiliation  

Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour ofS. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics ofSolanum aviculare cells immobilised within Ca-alginate beads were notably different from those of suspended cells. ImmobilisedS. aviculare have accumulated scopolin (up to 120 μg·g-1 FWB) within beads and excreted it into the culture medium (up to 8 μg·g-1 FWB). Contrary to suspended cells which have accumulated only traces of this metabolite within intracellular compartments (1 μg·g-1 FWB), no scopolin has been found into the culture medium.

中文翻译:

固定在钙藻酸盐凝胶珠粒中的茄果小球细胞增强了吗啡啉的产生。

通过改变钙(0.1至1.5M)和藻酸盐(1至1.5%)的浓度获得不同的基质,用于研究固定参数对S行为的影响。航空 已经观察到细胞生长,细胞释放以及scopolin产生和排泄的显着调节。固定在钙藻酸盐微珠中的茄属小球细胞的生理和形态特征与悬浮细胞明显不同。固定的 针叶中积累了scopolin(高达120μg·g-1 FWB)并将其排泄到培养基中(高达8μg·g-1 FWB)。与仅在细胞内区室(1μg·g-1 FWB)中积累了该代谢物痕迹的悬浮细胞相反,在培养基中未发现苏泊林。
更新日期:2019-11-01
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