Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.

中文翻译：

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采用纳米硅酸盐和 PEOT/PBT 3D 打印生物活性支架，用于骨组织工程。

增材制造 (AM) 在设计再生医学 3D 支架方面显示出了前景。然而，许多用于增材制造的合成生物材料都是生物惰性的。在这里，我们报告了由聚对苯二甲酸环氧乙烷（PEOT）/聚对苯二甲酸丁二醇酯（PBT）（PEOT/PBT）共聚物和2D纳米硅酸盐合成的生物活性纳米复合材料，用于制造骨组织工程的3D支架。PEOT/PBT 已被证明支持体内钙化和骨结合能力，而 2D 纳米硅酸盐在没有骨诱导剂的情况下诱导人间充质干细胞 (hMSC) 的成骨分化。研究了 PEOT/PBT 中添加纳米硅酸盐对结构、机械和生物性能的影响。具体来说，在 PEOT/PBT 中添加纳米硅酸盐可以提高纳米复合材料在生理条件下的稳定性，因为纳米硅酸盐抑制了共聚物的降解速率。然而，没有观察到由于添加纳米硅酸盐而导致支架的机械刚度显着增加。体外实验证明，在 PEOT/PBT 中添加纳米硅酸盐可改善 AM 纳米复合材料的生物活性。hMSC 很容易在含有纳米硅酸盐的支架上增殖，并导致骨相关蛋白的显着上调和矿化基质的产生。纳米硅酸盐和 PEOT/PBT 的协同能力可用于设计骨组织工程的生物活性支架。