In the field of tissue engineering the choice of materials is of great importance given the possibility to use biocompatible polymers produced by means of biotechnology. A large number of synthetic and natural materials have been used to this purpose and processed into scaffolds using Electrospinning technique. Among materials that could be used for the fabrication of scaffold and degradable membranes, natural polymers such as collagen, elastin or fibroin offer the possibility to design structures strictly similar to the extracellular matrix (ECM). Biotechnology and genetic engineering made possible the advent of a new class of biopolymers called protein-based polymers. One example is represented by the silk-elastin-proteins that combine the elasticity and resilience of elastin with the high tensile strength of silk-fibroin and display engineered bioactive sequences. In this work, we use electrospinning technique to produce a fibrous scaffold made of the co-recombinamer Silk-ELR. Obtained fibres have been characterized from the morphological point of view. Homogeneity and morphology have been explored using Scanning Electron Microscopy. A thorough study regarding the influence of Voltage, flow rate and distance have been carried out to determine the appropriate parameters to obtain the fibrous mats without defects and with a good distribution of diameters. Cytocompatibility has also been in vitro tested. For the first time we use the co-recombinamer Silk-ELR for the fabrication of a 2.5 angioplasty balloon coating. This structure could be useful as a coated scaffold for the regeneration of intima layer of vessels.

中文翻译：

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通过静电纺丝获得 Silk-ELR 共重组体覆盖支架。

在组织工程领域，考虑到使用通过生物技术生产的生物相容性聚合物的可能性，材料的选择非常重要。大量合成和天然材料已用于此目的，并使用静电纺丝技术加工成支架。在可用于制造支架和可降解膜的材料中，胶原蛋白、弹性蛋白或丝心蛋白等天然聚合物提供了设计与细胞外基质 (ECM) 严格相似的结构的可能性。生物技术和基因工程使得一类新型生物聚合物（称为蛋白质聚合物）的出现成为可能。丝弹性蛋白就是一个例子，它将弹性蛋白的弹性和弹性与丝素蛋白的高拉伸强度结合在一起，并显示出工程化的生物活性序列。在这项工作中，我们使用静电纺丝技术生产由共重组体 Silk-ELR 制成的纤维支架。所获得的纤维已从形态学的角度进行了表征。使用扫描电子显微镜探索了均匀性和形态。我们对电压、流量和距离的影响进行了深入研究，以确定获得无缺陷且直径分布良好的纤维垫的适当参数。细胞相容性也经过了体外测试。我们首次使用共重组体 Silk-ELR 来制造 2.5 血管成形球囊涂层。该结构可用作血管内膜层再生的涂层支架。