We have identified an IncX1 plasmid named pQJDSal1 from Salmonella enterica subsp. enterica serovar Pullorum (S. Pullorum). The plasmid is 67,685 bp in size and has 72 putative genes. pQJDSal1 harbors a conserved IncX1-type backbone with predicted regions for conjugation, replication and partitioning, as well as a toxin/antitoxin plasmid addiction system. Two regions (A and B) that have not been previously reported in IncX1 plasmids are inserted into the backbone. Region A (10.7 kb), inserted between parA and taxD, consists of a new Tn6168-like transposon containing an arsenic resistant operon arsB2CHR and sulfonamide resistance gene sul2. Region B contains another arsenic resistant operon arsADHR, resistance gene blaTEM-1B and three transposable elements. Conjugation experiments showed that pQJDSal1 could transfer from S. Pullorum to Escherichia coli (E. coli) J53. Statistical analysis of 70 sequenced IncX1 plasmids revealed that IncX1 plasmids harbored various antibiotic resistance genes. The results highlight the importance of IncX1 plasmids in disseminating antibiotic resistance genes.

中文翻译：

---

从肠沙门氏菌亚种分离的IncX1质粒。携带blaTEM-1B，sul2，抗砷操纵子的肠球菌白痢。

我们从肠沙门氏菌亚种中鉴定了一个名为pQJDSal1的IncX1质粒。小肠白痢（S. Pullorum）。该质粒大小为67,685 bp，具有72个推定基因。pQJDSal1包含一个保守的IncX1型骨架，具有预测的结合，复制和分配区域，以及毒素/抗毒素质粒成瘾系统。IncX1质粒中以前未报道的两个区域（A和B）被插入到骨架中。A区（10.7 kb），插入parA和taxD之间，由一个新的Tn6168样转座子组成，其中含有抗砷操纵子arsB2CHR和磺酰胺抗性基因sul2。B区包含另一个抗砷操纵子arsADHR，抗性基因blaTEM-1B和三个转座因子。结合实验表明pQJDSal1可以从S转移。鸡白痢对大肠杆菌（E. coli）J53。对70个测序的I​​ncX1质粒的统计分析表明，IncX1质粒具有多种抗生素抗性基因。结果突出了IncX1质粒在传播抗生素抗性基因中的重要性。