The use of lactic acid bacteria (LAB) to control multiple pathogens that affect different crops was studied, namely, Pseudomonas syringae pv. actinidiae in kiwifruit, Xanthomonas arboricola pv. pruni in Prunus and Xanthomonas fragariae in strawberry. A screening procedure based on in vitro and in planta assays of the three bacterial pathogens was successful in selecting potential LAB strains as biological control agents. The antagonistic activity of 55 strains was first tested in vitro and the strains Lactobacillus plantarum CC100, PM411 and TC92, and Leuconostoc mesenteroides CM160 and CM209 were selected because of their broad‐spectrum activity. The biocontrol efficacy of the selected strains was assessed using a multiple‐pathosystem approach in greenhouse conditions. L. plantarum PM411 and TC92 prevented all three pathogens from infecting their corresponding plant hosts. In addition, the biocontrol performance of PM411 and TC92 was comparable to the reference products (Bacillus amyloliquefaciens D747, Bacillus subtilis QST713, chitosan, acibenzolar‐S‐methyl, copper and kasugamycin) in semi‐field and field experiments. The in vitro inhibitory mechanism of PM411 and TC92 is based, at least in part, on a pH lowering effect and the production of lactic acid. Moreover, both strains showed similar survival rates on leaf surfaces. PM411 and TC92 can easily be distinguished because of their different multilocus sequence typing and random amplified polymorphic DNA profiles.

中文翻译：

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利用因其广谱活性而选择的植物乳杆菌菌株对细菌性植物病害进行生物防治

研究了利用乳酸菌（LAB）来控制影响不同作物的多种病原体，即丁香假单胞菌 pv。猕猴桃中的猕猴桃，Xanthomonas arboricola pv. 李子中的李子和草莓中的草莓黄单胞菌。基于三种细菌病原体的体外和植物内测定的筛选程序成功地选择了潜在的 LAB 菌株作为生物防治剂。首先对55株菌株的拮抗活性进行了体外测试，并选择植物乳杆菌CC100、PM411和TC92以及肠系膜明串珠菌CM160和CM209菌株，因为它们具有广谱活性。在温室条件下使用多病理系统方法评估所选菌株的生物防治功效。植物乳杆菌 PM411 和 TC92 阻止所有三种病原体感染其相应的植物宿主。此外，在半田和田间实验中，PM411和TC92的生防性能与参考产品（解淀粉芽孢杆菌D747、枯草芽孢杆菌QST713、壳聚糖、苯并-S-甲基、铜和春雷霉素）相当。PM411 和 TC92 的体外抑制机制至少部分基于 pH 降低作用和乳酸的产生。此外，两种菌株在叶表面的存活率相似。PM411 和 TC92 因其不同的多位点序列分型和随机扩增的多态性 DNA 谱而很容易区分。