A toxicoproteomic study was performed on liver of rats treated with retrorsine (RTS), a representative hepatotoxic pyrrolizidine alkaloid at a toxic dose (140 mg/kg) known to cause severe acute hepatotoxicity. By comparing current data with our previous findings in mild liver lesions of rats treated with a lower dose of RTS, seven proteins and three toxicity pathways of vascular endothelial cell death, which was further verified by observed sinusoidal endothelial cell losses, were found uniquely associated with retrorsine-induced hepatotoxicity. This toxicoproteomic study of acute pyrrolizidine alkaloid intoxication lays a foundation for future investigation to delineate molecular mechanisms of pyrrolizidine alkaloid-induced hepatotoxicity.

中文翻译：

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毒理学评估大鼠对吡咯并立啶生物碱急性中毒的反应。

用逆转录酶（RTS）治疗大鼠的肝脏进行了毒物学研究，逆转录酶（RTS）是一种具有代表性的肝毒性吡咯烷核生物碱，毒性剂量为140 mg / kg，已知会引起严重的急性肝毒性。通过将当前数据与我们先前在使用较低剂量的RTS治疗的大鼠轻度肝损伤中的发现进行比较，发现血管内皮细胞死亡的7种蛋白质和3种毒性途径（通过观察到的窦状内皮细胞损失进一步证实）与逆转录酶诱导的肝毒性。急性吡咯烷定生物碱中毒的这项毒物学研究为进一步研究勾勒吡咯烷定生物碱诱导的肝毒性的分子机制奠定了基础。