The increased worldwide mortality rate due to liver cancer may be attributed to the aggressive nature of the disease. Signal transduction through G-protein-coupled receptors (GPCRs) can affect a number of aspects of cancer biology, including invasion, migration and vascular remodelling. JTC-801, a novel GPCR antagonist, has demonstrated promising anticancer effects in adenocarcinoma and osteosarcoma cells. In the present study, the effect of JTC-801 on the proliferation and migration of hepatoblastoma Hep G2 cells was investigated. The Cell Counting Kit-8 assay revealed that JTC-801 markedly suppressed the growth of the Hep G2 cells. Additionally, JTC-801 significantly inhibited cell invasion and migration in a Transwell assay. Furthermore, the expression of anti-apoptotic protein B-cell lymphoma 2 decreased and the expression of the pro-apoptotic proteins active caspase-3 and apoptosis regulator BAX increased in the Hep G2 cells following JTC-801 treatment. Additionally, JTC-801 suppressed the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signalling pathway in the Hep G2 cells. Therefore, the present study revealed that JTC-801 can induce the apoptosis of Hep G2 cells by regulating the PI3K/AKT signalling pathway, which suggests that JTC-801 may be a potential novel drug target for clinical liver cancer treatment.

中文翻译：

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JTC-801通过调节磷脂酰肌醇3激酶/蛋白激酶B信号通路抑制Hep G2肝母细胞瘤细胞系的增殖和转移。

由于肝癌而导致的全球死亡率增加可能归因于该疾病的侵略性。通过G蛋白偶联受体（GPCR）进行的信号转导可以影响癌症生物学的许多方面，包括入侵，迁移和血管重塑。JTC-801是一种新型GPCR拮抗剂，已在腺癌和骨肉瘤细胞中显示出有希望的抗癌作用。在本研究中，研究了JTC-801对肝母细胞瘤Hep G2细胞增殖和迁移的影响。Cell Counting Kit-8分析显示JTC-801显着抑制了Hep G2细胞的生长。另外，在Transwell分析中，JTC-801显着抑制了细胞的侵袭和迁移。此外，JTC-801处理后，Hep G2细胞中抗凋亡蛋白B细胞淋巴瘤2的表达降低，促凋亡蛋白活性caspase-3和凋亡调节剂BAX的表达增加。此外，JTC-801抑制了Hep G2细胞中的磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）信号通路。因此，本研究表明，JTC-801可以通过调节PI3K / AKT信号通路来诱导Hep G2细胞凋亡，这表明JTC-801可能是临床肝癌治疗的潜在新药靶点。