Besides liver, IGF-I is expressed in adipose tissue. However, the effects of this local IGF-I on adipose tissue and metabolism are unclear. We generated adipocyte-specific knock-out mice on the background of the Berlin Fat Mouse Inbred (BFMI) line to evaluate the contribution of adipocyte-IGF-I on glucose metabolism and adipose tissue development. BFMI mice are obese, non-diabetic with elevated plasma insulin and IGF-I concentration. The knock-out in adipocytes led to a total white adipose tissue expression of 50–60% due to unaltered Igf-1 expression in stromavascular cells. The lack of IGF-I from adipocytes did not alter plasma IGF-I concentration. BFMI^Chr3-Igf-I-KO^Q-AT mice had reduced adipose tissue mass in most depots. During oral glucose tolerance tests, BFMI^Chr3-Igf-I-KO^Q-AT mice showed an impaired glucose clearance (p = .03). Interestingly, insulin action was enhanced during insulin tolerance tests (p = .05). In conclusion, adipocyte-specific IGF-I ablation in obese BFMI mice results in reduced adipose tissue mass and thereby alters glucose metabolism.

除肝脏外，IGF-I在脂肪组织中表达。然而，尚不清楚该局部IGF-1对脂肪组织和代谢的影响。我们在柏林肥胖小鼠近交系（BFMI）的背景上生成了脂肪细胞特异性的敲除小鼠，以评估脂肪细胞-IGF-1对葡萄糖代谢和脂肪组织发育的贡献。BFMI小鼠是肥胖，非糖尿病且血浆胰岛素和IGF-I浓度升高。由于基质血管细胞中Igf-1的表达没有改变，脂肪细胞的剔除导致白色脂肪组织的总表达达到50-60％。脂肪细胞缺乏IGF-1不会改变血浆IGF-1浓度。BFMI ^Chr3 -Igf-I-KO ^Q-AT在大多数仓库中，小鼠的脂肪组织量减少。在口服葡萄糖耐量试验期间，BFMI ^Chr3 -Igf-I-KO ^Q-AT小鼠显示葡萄糖清除受损（p  = .03）。有趣的是，在胰岛素耐受性测试中，胰岛素作用得到了增强（p  = 0.05）。总之，肥胖BFMI小鼠的脂肪细胞特异性IGF-I消融可导致脂肪组织量减少，从而改变葡萄糖代谢。