In mammals, preovulatory oocytes are encircled by several layers of granulosa cells (GCs) in follicular microenvironment. These follicular oocytes are arrested at diplotene arrest due to high level of cyclic nucleotides from encircling GCs. Pituitary gonadotropin acts at the level of encircling GCs and increases adenosine 3′,5′-cyclic monophosphate (cAMP) and guanosine 3′,5′-cyclic monophosphate (cGMP) and activates mitogen-activated protein kinase 3/1 (MAPK3/1) signaling pathway. The MAPK3/1 disrupts the gap junctions between encircling GCs and oocyte. The disruption of gap junctions interrupts the transfer of cyclic nucleotides to the oocyte that results a drop in intraoocyte cAMP level. A transient decrease in oocyte cAMP level triggers maturation promoting factor (MPF) destabilization. The destabilized MPF finally triggers meiotic resumption from diplotene arrest in follicular oocyte. Thus, MAPK3/1 from GCs origin plays important role in gonadotropin-mediated meiotic resumption from diplotene arrest in follicular oocyte of mammals.

在哺乳动物中，在卵泡微环境中，排卵前的卵母细胞被几层颗粒细胞（GC）包围。这些卵泡卵母细胞由于来自周围GC的高水平环状核苷酸而被捕获在二烯烯捕获中。垂体促性腺激素在环绕GC的水平上起作用并增加腺苷3'，5'-环一磷酸（cAMP）和鸟苷3'，5'-环一磷酸（cGMP）并激活促分裂原激活的蛋白激酶3/1（MAPK3 / 1 ）信号通路。MAPK3 / 1破坏了周围的GC和卵母细胞之间的间隙连接。间隙连接的破坏中断了环核苷酸向卵母细胞的转移，导致卵内cAMP水平下降。卵母细胞中cAMP水平的短暂降低会触发成熟促进因子（MPF）不稳定。不稳定的MPF最终触发了卵泡卵母细胞中双戊烯阻滞导致减数分裂的恢复。因此，来自GC的MAPK3 / 1在促性腺激素介导的减数分裂从哺乳动物卵泡卵母细胞中的二倍体逮捕中起重要作用。