Supplementing suckling piglets with Lactobacillus reuteri isolated from a homologous source improves L. reuteri colonization number in the gastrointestinal tract, which can have health benefits. This study investigated dietary L. reuteri supplementation on the growth and health—including immune status—of piglets, as well as its colonization. A total of 60 sows with similar parity and body weight were allocated into one of three groups after secretion (n = 20 each, with 10 neonatal piglets of each): untreated control, L. reuteri supplementation, and antibiotic treatment. The experimental duration was 28 days, from birth of piglets to their group transferred. For the first 7 days after birth, all neonatal piglets were fed by sows. Piglets in the L. reuteri supplementation group were administered with 1.0 ml L. reuteri fermentation broth containing 5.0 × 10⁷ CFU. From 7 to 28 days, piglets were given basal feed (control), basal feed supplemented with L. reuteri (1.0 × 10⁷ CFU/g), or aureomycin (150 mg/kg). L. reuteri colonization in the distal jejunum and ileum was increased in piglets in the L. reuteri-supplemented as compared to the control group after 28 days, as determined by fluorescence in situ hybridization and real-time PCR analysis. Total Lactobacillus and Bifidobacterium counts in the cecum were higher whereas total aerobic bacteria (Escherichia coli and Staphylococcus) counts were lower in the L. reuteri as compared to the control group. L. reuteri supplementation also improved body antioxidant status and immune function relative to control animals. Strain-specific L. reuteri administered to piglets colonizes the intestinal mucosa and improves cecal microbiota profile and whole-body antioxidant and immune status, leading to better growth and lower morbidity and mortality rates.

中文翻译：

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罗伊氏乳杆菌KT260178补充剂通过有针对性的定殖，改善盲肠微生物群和免疫功能而降低了仔猪的发病率。

用从同源来源分离出的罗伊氏乳杆菌补充乳猪可改善罗伊氏乳杆菌在胃肠道中的定殖数，这可能对健康有益。这项研究调查了膳食补充罗伊氏乳杆菌对仔猪的生长和健康（包括免疫状况）及其定植的影响。分泌后，将总共60头具有相同胎龄和体重的母猪分为三组（ 每组n = 20，每组10头新生仔猪）：未经治疗的对照，罗伊氏乳杆菌补充和抗生素治疗。实验持续时间为28天，从仔猪出生到他们的小组转移。出生后的前7天，所有新生仔猪均以母猪喂养。补充罗伊氏乳杆菌组的仔猪，加入1.0 ml 含5.0×10 ⁷  CFU的罗伊氏乳杆菌发酵液。从7到28天，给仔猪饲喂基础饲料（对照组），补充罗伊氏乳杆菌（1.0×10 ⁷  CFU / g）或金霉素（150 mg / kg）的基础饲料。罗伊氏乳杆菌在空肠远端殖民化和回肠仔猪在增加罗伊氏乳杆菌通过荧光原位杂交和实时PCR分析确定，在28天后与对照组相比-补充。与对照组相比，盲肠乳酸杆菌和双歧杆菌的总计数较高，而罗伊氏乳杆菌的总有氧细菌（大肠杆菌和葡萄球菌）计数较低。相对于对照动物，补充罗伊氏乳杆菌还改善了机体抗氧化状态和免疫功能。菌株特异性罗伊氏乳杆菌 对仔猪施用后，可在肠道粘膜上定殖，并改善盲肠微生物群以及整个人体的抗氧化和免疫状态，从而促进生长，降低发病率和死亡率。