Reseeding of decellularized organ scaffolds with a patient's own cells has promise for eliminating graft versus host disease. This study investigated whether ultrasound imaging or magnetic resonance imaging (MRI) can track the reseeding of murine liver scaffolds with silica-labeled or iron-labeled liver hepatocytes. Mesoporous silica particles were created using the Stöber method, loaded with Alexa Flour 647 fluorophore, and conjugated with protamine sulfate, glutamine, and glycine. Fluorescent iron oxide particles were obtained from a commercial source. Liver cells from donor mice were loaded with the silica particles or iron oxide particles. Donor livers were decellularized and reperfused with silica-labeled or iron-labeled cells. The reseeded livers were longitudinally analyzed with ultrasound imaging and MRI. Liver biopsies were imaged with confocal microscopy and scanning electron microscopy. Ultrasound imaging had a detection limit of 0.28 mg/mL, while MRI had a lower detection limit of 0.08 mg/mL based on particle weight. The silica-loaded cells proliferated at a slower rate compared to iron-loaded cells. Ultrasound imaging, MRI, and confocal microscopy underestimated cell numbers relative to scanning electron microscopy. Ultrasound imaging had the greatest underestimation due to coarse resolution compared to the other imaging modalities. Despite this underestimation, both ultrasound imaging and MRI successfully tracked the longitudinal recellularization of liver scaffolds.

中文翻译：

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氧化铁颗粒和二氧化硅颗粒用于追踪器官再细胞化的比较。

将脱细胞的器官支架与患者自身细胞一起播种有望消除移植物抗宿主疾病。这项研究调查了超声成像或磁共振成像（MRI）是否可以跟踪带有硅胶标记或铁标记的肝肝细胞的鼠肝支架的再播种。使用Stöber方法制得中孔二氧化硅颗粒，该颗粒中装有Alexa Flour 647荧光团，并与硫酸鱼精蛋白，谷氨酰胺和甘氨酸偶联。荧光氧化铁颗粒获自商业来源。将来自供体小鼠的肝细胞装载二氧化硅颗粒或氧化铁颗粒。将供体肝脏脱细胞并用二氧化硅标记或铁标记的细胞再灌注。用超声成像和MRI对重播的肝脏进行纵向分析。用共聚焦显微镜和扫描电子显微镜对肝脏活组织检查成像。超声成像的检出限为0.28 mg / mL，而MRI的检出限为0.08 mg / mL（基于颗粒重量）。与装载铁的细胞相比，装载硅石的细胞增殖速度较慢。相对于扫描电子显微镜，超声成像，MRI和共聚焦显微镜低估了细胞数量。与其他成像方式相比，超声成像由于分辨率较差而被最大程度地低估了。尽管低估了这一点，但是超声成像和MRI都成功地追踪了肝支架的纵向细胞再形成。08 mg / mL（基于粒径）。与铁负载的细胞相比，二氧化硅负载的细胞以较慢的速度增殖。相对于扫描电子显微镜，超声成像，MRI和共聚焦显微镜低估了细胞数量。与其他成像方式相比，超声成像由于分辨率较差而被最大程度地低估了。尽管低估了这一点，但是超声成像和MRI都成功地追踪了肝支架的纵向细胞再形成。08 mg / mL（基于粒径）。与装载铁的细胞相比，装载硅石的细胞增殖速度较慢。相对于扫描电子显微镜，超声成像，MRI和共聚焦显微镜低估了细胞数量。与其他成像方式相比，超声成像由于分辨率较差而被最大程度地低估了。尽管低估了这一点，但是超声成像和MRI都成功地追踪了肝支架的纵向细胞再形成。