During surveys of Phytophthora diversity in natural and semi-natural Fagaceae forests in Austria, Italy and Portugal, four new cryptic species were isolated from rhizosphere soil samples. Multigene phylogeny based on nuclear ITS, ß-tubulin and HSP90 and mitochondrial cox1 and NADH1 gene sequences demonstrated that two species, P. tyrrhenica and P. vulcanica spp. nov., belong to phylogenetic Clade 7a, while the other two species, P. castanetorum and P. tubulina spp. nov., clustered together with P. quercina forming a new clade, named here as Clade 12. All four new species are homothallic and have low optimum and maximum temperatures for growth and very slow growth rates at their respective optimum temperature. They differed from each other and from related species by a unique combination of morphological characters, cardinal temperatures, and growth rates. Pathogenicity of all Phytophthora species to the root system of their respective host species was demonstrated in soil infestation trials.

中文翻译：

---

来自奥地利、意大利和葡萄牙壳斗科森林的多种新的隐匿致病疫霉属物种。


在对奥地利、意大利和葡萄牙的天然和半天然壳斗科森林中的疫霉菌多样性进行调查期间，从根际土壤样本中分离出了四种新的隐秘物种。基于核 ITS、β-微管蛋白和 HSP90 以及线粒体 cox1 和 NADH1 基因序列的多基因系统发育表明，P. tyrhenica 和 P. vulcanica spp 两个物种。 11 月，属于系统发育分支 7a，而其他两个物种 P.castanetorum 和 P.tubulina spp. 属于系统发育分支 7a。 11 月，与 P. quercina 聚集在一起形成一个新的分支，此处命名为 Clade 12。所有四个新物种都是同宗的，并且具有较低的最适和最高生长温度，并且在各自的最适温度下生长速率非常慢。它们彼此之间以及与相关物种的不同之处在于形态特征、基本温度和生长速度的独特组合。所有疫霉属物种对其各自宿主物种根系的致病性均在土壤侵染试验中得到证实。