Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.

中文翻译：

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实时荧光定量PCR在加工食品中的坚果过敏原检测中的应用。

当前，食物过敏是全世界重要的健康问题。食品加工过程中由于意外污染而导致存在未声明的致敏成分或痕量过敏原，对致敏个体构成巨大的健康风险。因此，需要可靠的分析方法来检测和识别食品中的致敏成分。实时PCR可以特异性和准确地扩增变应原序列。一些加工方法可能诱导基因组DNA的断裂和/或降解，并且已经进行了一些研究来分析加工在检测和不施加压力的情况下对不同目标的检测效果，例如热处理。在这篇评论中 我们提供了实时PCR在检测加工食品中坚果过敏原方面的应用的最新概述。还回顾和讨论了有助于变应原检测的PCR方法性能的不同变量。