Seriola quinqueradiata (yellowtail) is the most widely farmed and economically important fish in aquaculture in Japan. In this study, we used the genome of haploid yellowtail fish larvae for de novo assembly of whole-genome sequences, and built a high-quality draft genome for the yellowtail. The total length of the assembled sequences was 627.3 Mb, consisting of 1,394 scaffold sequences (>2 kb) with an N50 length of 1.43 Mb. A total of 27,693 protein-coding genes were predicted for the draft genome, and among these, 25,832 predicted genes (93.3%) were functionally annotated. Given our lack of knowledge of the yellowtail digestive system, and using the annotated draft genome as a reference, we conducted an RNA-Seq analysis of its three digestive organs (stomach, intestine and rectum). The RNA-Seq results highlighted the importance of certain genes in encoding proteolytic enzymes necessary for digestion and absorption in the yellowtail gastrointestinal tract, and this finding will accelerate development of formulated feeds for this species. Since this study offers comprehensive annotation of predicted protein-coding genes, it has potential broad application to our understanding of yellowtail biology and aquaculture.

中文翻译：

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消化道的黄尾鱼（Seriola quinqueradiata）基因组和转录组图谱。

Seriola quinqueradiata（黄尾鱼）是日本水产养殖中养殖最广泛且经济上重要的鱼类。在这项研究中，我们将单倍体yellow鱼幼虫的基因组用于整个基因组序列的从头组装，并建立了高质量的draft鱼草图基因组。组装序列的总长度为627.3 Mb，由1,394个支架序列（> 2 kb）组成，N50长度为1.43 Mb。共有27,693个蛋白质编码基因被编码用于草图基因组，其中25,832个预测基因（93.3％）被功能注释。鉴于我们缺乏对tail鱼消化系统的了解，并使用带注释的基因组草图作为参考，我们对其三个消化器官（胃，肠和直肠）进行了RNA-Seq分析。RNA-Seq结果突显了某些基因在编码鱼尾胃肠道消化和吸收所必需的蛋白水解酶中的重要性，这一发现将加快该物种配方饲料的开发。由于这项研究为预测​​的蛋白质编码基因提供了全面的注释，因此它对我们对yellow鱼生物学和水产养殖的理解具有潜在的广泛应用。