Starch present in the endosperm of common wheat (Triticum aestivum L.) grains is an important source of carbohydrates worldwide. Starches with a greater proportion of amylose have increased levels of resistant starch, a dietary fiber that can provide human health benefits. Induced mutations in STARCH BRANCHING ENZYME II (SBEII) genes in wheat are associated with increased amylose and resistant starch. Ethyl methane sulfonate mutations in SBEIIa and SBEIIb paralogs were combined in the hexaploid wheat cultivar Lassik. Four mutant combinations were generated: SBEIIa/b‐AB (Reg. No. GP‐997, PI 675644); SBEIIa/b‐A, SBEIIa‐D (Reg. No. GP‐998, PI 675645); SBEIIa/b‐B, SBEIIa‐D (Reg. No. GP‐999, PI 675646); and SBEIIa/b‐AB, SBEIIa‐D (Reg. No. GP‐1000, PI 675647). The SBEII mutant lines were compared with a wild‐type control in a greenhouse and field experiment. The quintuple mutant line (SBEIIa/b‐AB, SBEIIa‐D) presented significant increases in both amylose (51% greenhouse; 63% field) and resistant starch (947% greenhouse; 1057% field) relative to the control. A decrease in total starch content (7.8%) was observed in the field experiment. The quintuple mutant also differed in starch viscosity parameters. Registration of the hexaploid wheat SBEII‐mutant lines by University of California, Davis can help expedite the development of common wheat cultivars with increased amylose and resistant starch content.

中文翻译：

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具有 SBEII 基因突变的普通小麦种质的登记，赋予谷物直链淀粉和抗性淀粉含量增加。


普通小麦（ Triticum aestivum L.）胚乳中存在的淀粉是全世界碳水化合物的重要来源。直链淀粉比例较高的淀粉会增加抗性淀粉的含量，抗性淀粉是一种可为人类健康带来益处的膳食纤维。小麦中淀粉分支酶 II ( SBEII ) 基因的诱导突变与直链淀粉和抗性淀粉的增加有关。 SBEIIa和SBEIIb旁系同源物中的甲基磺酸乙酯突变在六倍体小麦品种 Lassik 中结合在一起。生成了四种突变体组合： SBEIIa/b -AB（注册号 GP-997，PI 675644）； SBEIIa/b- A、 SBEIIa -D（注册号 GP-998，PI 675645）； SBEIIa/b ‐B、 SBEIIa ‐D（注册号 GP‐999，PI 675646）；和SBEIIa/b ‐AB、 SBEIIa ‐D（注册号 GP‐1000，PI 675647）。在温室和田间实验中将SBEII突变系与野生型对照进行比较。与对照相比，五重突变系（ SBEIIa/b -AB、 SBEIIa -D）的直链淀粉（51% 温室；63% 田间）和抗性淀粉（947% 温室；1057% 田间）均显着增加。田间实验观察到总淀粉含量下降（7.8%）。五元突变体的淀粉粘度参数也不同。加州大学戴维斯分校注册的六倍体小麦SBEII突变体系有助于加快直链淀粉和抗性淀粉含量增加的普通小麦品种的开发。