Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

中文翻译：

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化学蛋白质组学中的光亲和标记（PAL）：研究蛋白质-蛋白质相互作用（PPI）的便捷工具。

蛋白质间相互作用（PPI）触发了广泛的生物信号通路，这些通路对于生物医学研究和药物发现至关重要。已经使用了各种技术来研究特定的蛋白质，包括亲和层析，基于活性的探针，基于亲和力的探针和光亲和标记（PAL）。PAL已成为研究PPI的最强大策略之一。PAL中使用了传统的光交联剂，包括二苯甲酮，芳基叠氮化物和重氮。在光辐照下，这些光交联剂（Pls）产生与相邻分子反应的高反应性物质，从而导致直接的共价修饰。这篇综述介绍了使用PAL进行PPI的化学蛋白质组学研究的最新实例。