Exposure to organophosphate (OP) compounds leads to behavioral alterations. To determine whether paraoxon has effects on anxiety, anxiety-like behaviors were assessed in paraoxon-exposed rats. Protein expression of glutamate transporters has also been measured in hippocampus and prefrontal cortex. Three doses of paraoxon (0.3, 0.7, or 1 mg/kg) or corn oil (vehicle) were intraperitoneally injected to adult male rats. At 14 or 28 days after exposure, behavioral tests were done using elevated plus-maze (EPM) or open field tests. Thereafter, animals were sacrificed and both hippocampi and prefrontal cortices were extracted for cholinesterase assay and western blotting. Animals treated with convulsive doses of paraoxon (0.7 and 1 mg/kg) showed an increase in percentage of time spent in open arms and percentage of open arm entries in the EPM. In the open field test, an increase in the time spent in central area was observed in rats treated with the same doses of paraoxon. These effects of paraoxon were independent of any changes in locomotor activity. There was an increase in both astrocytic glutamate transporter proteins (GLAST and GLT-1) in the hippocampus of animals treated with 0.7 and 1 mg/kg of paraoxon. In the prefrontal cortex, protein levels of the GLAST and GLT-1 increased in 0.7 and decreased in 1 mg/kg groups. Only a significant decrease in EAAC1 protein was observed in the prefrontal cortex at 14 days following exposure to 1 mg/kg of paraoxon. Collectively, this study showed that exposure to convulsive doses of paraoxon induced anxiolytic-like behaviors in both behavioral tests. This effect may be attributed to alterations of glutamate transporter proteins in the rat hippocampus and prefrontal cortex.

中文翻译：

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对氧磷的抗焦虑活性与大​​鼠脑谷氨酸能系统的改变有关。

暴露于有机磷酸酯（OP）化合物会导致行为改变。为了确定对氧磷是否对焦虑有影响，在对氧磷暴露的大鼠中评估了焦虑样行为。谷氨酸转运蛋白的蛋白质表达也已在海马和前额叶皮层中进行了测量。向成年雄性大鼠腹膜内注射三剂对氧磷（0.3、0.7或1 mg / kg）或玉米油（载体）。暴露后14或28天，使用高架迷宫（EPM）或露天测试进行行为测试。此后，处死动物并提取海马和前额叶皮层用于胆碱酯酶测定和蛋白质印迹。用惊厥剂量的对氧磷（0.7和1 mg / kg）治疗的动物在EPM中的张开双臂时间和张开双臂进入的百分比增加。在野外试验中，在用相同剂量的对氧磷处理的大鼠中，观察到在中心区域花费的时间增加了。对氧磷的这些作用与运动活动的任何变化无关。用0.7和1 mg / kg对氧磷处理的动物海马中的星形胶质谷氨酸转运蛋白（GLAST和GLT-1）均增加。在前额叶皮层中，GLAST和GLT-1的蛋白质水平在0.7中增加，在1 mg / kg组中降低。暴露于1 mg / kg对氧磷后14天，仅在额叶前额皮层中观察到EAAC1蛋白的显着降低。总体而言，这项研究表明，在两种行为测试中，惊厥剂量的对氧磷暴露均可引起抗焦虑样行为。