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FISH-based karyotyping of Pelmatohydraoligactis (Pallas, 1766), Hydraoxycnida Schulze, 1914, and H.magnipapillata Itô, 1947 (Cnidaria, Hydrozoa).
Comparative Cytogenetics ( IF 1.0 ) Pub Date : 2019-01-08 , DOI: 10.3897/compcytogen.v12i2.32120
Boris A Anokhin 1 , Valentina G Kuznetsova 1
Affiliation  

An account is given of the karyotypes of Hydramagnipapillata Itô, 1947, H.oxycnida Schulze, 1914, and Pelmatohydraoligactis (Pallas, 1766) (Cnidaria, Hydrozoa, Hydridae). A number of different techniques were used: conventional karyotype characterization by standard staining, DAPI-banding and C-banding was complemented by the physical mapping of the ribosomal RNA (18S rDNA probe) and H3 histone genes, and the telomeric (TTAGGG) n sequence by fluorescence in situ hybridization (FISH). We found that the species studied had 2n = 30; constitutive heterochromatin was present in the centromeric regions of the chromosomes; the "vertebrate" telomeric (TTAGGG) n motif was located on both ends of each chromosome and no interstitial sites were detected; 18S rDNA was mapped on the largest chromosome pair in H.magnipapillata and on one of the largest chromosome pairs in H.oxycnida and P.oligactis; in H.magnipapillata, the major rRNA and H3 histone multigene families were located on the largest pair of chromosomes, on their long arms and in the centromeric areas respectively. This is the first chromosomal mapping of H3 in hydras.

中文翻译:

Pelmatohydraoligactis(帕拉斯,1766),Hydraoxycnida舒尔茨,1914年,和H.magnipapillata ITO,1947(刺胞动物门,水螅纲)的基于FISH的核型分析。

帐户被给出Hydramagnipapillata ITO,1947年,H.oxycnida舒尔茨,1914年,和Pelmatohydraoligactis(帕拉斯,1766)(刺胞动物门,水螅纲,Hydridae)的核型。许多不同的技术被用来:通过标准染色,DAPI显带和C带常规核型表征是由核糖体RNA的物理作图(18S rDNA的探针)和H3组蛋白基因,以及端粒(TTAGGG)N序列互补原位杂交(FISH)的荧光。我们发现,研究物种必须为2n = 30; 结构异存在于染色体的着丝粒区; 了“脊椎动物”端粒(TTAGGG)N基序位于每个染色体的两端,并且无间隙位置进行检测; 18S rDNA序列被定位在H.最大的染色体对 magnipapillata以及在H.oxycnida和P.oligactis最大的对染色体之一; 在H.magnipapillata,主要rRNA和H3组蛋白的多基因家族分别位于最大的对染色体,他们的长武器,分别着丝粒区域。这是水螅H3的第一个染色体定位。
更新日期:2019-11-01
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