The possibility of developing novel contrast imaging agents for cancer cellular labelling and fluorescence imaging applications were explored using silica-coated cadmium selenide (CdSe) quantum dots (QDs). The time dependent cellular internalization efficiency study was carried out using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and Confocal Laser Scanning Microscopy (cLSM) after exposing QDs to stem cells and cancer cells. The strong fluorescence from the cytoplasm confirmed that the QDs were efficiently internalized by the cells. The internalization maxima were observed at the fourth hour of incubation in both stem and cancer cells. Further, the in vitro fluorescence imaging as well as localization study of QDs were performed in various cells. Moreover, high contrast in vivo tumor imaging efficiency of silica-coated CdSe QDs was performed in ultrathin sections of tumor mice, and the results confirmed its effective role in cellular imaging and labelling in cancer and other diseases.

中文翻译：

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二氧化硅涂层的CdSe量子点的有效细胞内在化，可用于高对比度癌症成像和标记应用。

使用二氧化硅涂层的硒化镉（CdSe）量子点（QD）探索了开发用于癌细胞标记和荧光成像应用的新型造影剂的可能性。在将量子点暴露于干细胞和癌细胞后，使用感应耦合等离子体发射光谱法（ICP-OES）和共聚焦激光扫描显微镜（cLSM）进行了时间依赖性细胞内化效率研究。来自细胞质的强荧光证实了量子点被细胞有效地内在化了。在培养的第四小时，在干细胞和癌细胞中均观察到内化最大值。此外，在各种细胞中进行了体外荧光成像以及量子点的定位研究。此外，