Five species belonging to the genus Gampsocleis Fieber, 1852 were analyzed using fluorescence in situ hybridization (FISH) with 18S rDNA and telomeric probes, as well as C-banding, DAPI/CMA3 staining and silver impregnation. The studied species showed two distinct karyotypes, with 2n = 31 (male) and 2n = 23 (male) chromosomes. The drastic reduction in chromosome number observed in the latter case suggests multiple translocations and fusions as the main responsible that occurred during chromosome evolution. Two groups of rDNA distribution were found in Gampsocleis representatives analyzed. Group 1, with a single large rDNA cluster on the medium-sized autosome found in four species, carried in the haploid karyotype. Group 2, represented only by G.abbreviata, was characterized by the presence of two rDNA signals. TTAGG telomeric repeats were found at the ends of chromosome arms as expected. The rDNA clusters coincided with active NORs and GC-rich segments.

中文翻译：

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对Gampsocleis属（直翅目，Tettigoniinae，Gampsocleidini）的核型进化的新见解。

使用18S rDNA和端粒探针的荧光原位杂交（FISH）以及C带，DAPI / CMA3染色和银浸渍技术，分析了1852年属于Gampsocleis Fieber属的5个种。研究的物种显示出两种不同的核型，分别具有2n = 31（雄性）和2n = 23（雄性）的染色体。在后一种情况下观察到的染色体数目的急剧减少表明染色体易位和融合是染色体进化过程中发生的主要原因。在Gampsocleis代表中发现了两组rDNA分布。第一组，单倍核型携带，在四个物种的中等大小的常染色体上具有单个大的rDNA簇。仅由G.abbreviata代表的第2组的特征是存在两个rDNA信号。如预期的那样，在染色体臂的末端发现了TTAGG端粒重复序列。rDNA簇与活跃的NOR和富含GC的片段相吻合。