Background

To evaluate changes in total choline (tCho) absolute concentration ([tCho]) in the breast of healthy fertile women in relation to menstrual cycle (MC) or use of oral contraceptives (OC).

Methods

After institutional review board approval, we prospectively evaluated 40 healthy fertile volunteers: 20 with physiological MC, aged 28 ± 3 years (mean ± standard deviation; nOC group); 20 using OC, aged 26 ± 3 years (OC group). Hormonal assays and water-suppressed single-voxel 3-T proton magnetic resonance spectroscopy (¹H-MRS) were performed on MC days 7, 14, and 21 in the nOC group and only on MC day 14 in the OC group. [tCho] was measured versus an external phantom. Mann-Whitney U test and Spearman coefficient were used; data are given as median and interquartile interval.

Results

All spectra had good quality. In the nOC group, [tCho] (mM) did not change significantly during MC: 0.8 (0.3–2.4) on day 7, 0.9 (0.4–1.2) on day 14, and 0.4 (0.2–0.8) on day 21 (p = 0.963). In the OC group, [tCho] was 0.7 (0.2–1.7) mM. The between-groups difference was not significant on all days (p ≥ 0.411). All hormones except prolactin changed during MC (p ≤ 0.024). In the OC group, [tCho] showed a borderline correlation with estradiol (r = 0.458, p = 0.056), but no correlation with other hormones (p ≥ 0.128). In the nOC group, [tCho] negatively correlated with prolactin (r = -0.587, p = 0.006) on day 7; positive correlation was found with estradiol on day 14 (r = 0.679, p = 0.001).

Conclusions

A tCho peak can be detected in the normal mammary gland using 3-T ¹H-MRS. The [tCho] in healthy volunteers was 0.4–0.9 mM, constant over the MC and independent of OC use.

中文翻译：

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健康可育年轻女性乳房中总胆碱浓度与月经周期或口服避孕药有关的变化：一项3-T 1H-MRS研究。

背景

为了评估与月经周期（MC）或口服避孕药（OC）有关的健康可育妇女的乳房中总胆碱（tCho）绝对浓度（tCho）的变化。

方法

经过机构审查委员会的批准，我们对40名健康的可育志愿者进行了前瞻性评估：20名具有生理MC的受试者，年龄28±3岁（平均±标准差； nOC组）；使用OC的20位患者，年龄26±3岁（OC组）。在nOC组的MC第7、14和21天进行激素测定和水抑制的单体素3-T质子磁共振波谱（¹ H-MRS），而在OC组仅在MC第14天进行激素测定和水抑制单体素3T质子磁共振波谱。测量了相对于外部体模的tCho。使用Mann-Whitney U检验和Spearman系数；数据以中位数和四分位数间隔给出。

结果

所有光谱均具有良好的质量。在nOC组中，MC期间的tCho（mM）没有显着变化：第7天为0.8（0.3-2.4），第14天为0.9（0.4-1.2），第21天为0.4（0.2-0.8）（p  = 0.963）。在OC组中，tCho为0.7（0.2–1.7）mM。组间差异并不在所有天（显著p  ≥0.411）。除了催乳素所有激素MC过程中改变（p  ≤0.024）。在OC组，[TCHO]显示出与雌二醇（边界线的相关性[R  = 0.458，p  = 0.056），但与其他激素（无相关性p  ≥0.128）。在nOC组中，tCho与催乳素呈负相关（r  = -0.587，p = 0.006）在第7天；在第14天发现与雌二醇呈正相关（r  = 0.679，p  = 0.001）。

结论

可以使用3-T ¹ H-MRS在正常乳腺中检测到tCho峰。健康志愿者的tCho为0.4-0.9 mM，在MC上恒定，与OC的使用无关。