Multispectral photoacoustic tomography provides mapping of the tissue chromophore distributions using sets of tunable laser wavelengths. With the overall goal of studying the dynamics of cerebrospinal fluid in mice in vivo, our work aims to minimize the number of wavelengths to reduce scanning time, improve the temporal resolution, reduce the energy deposition and avoid the tracer photobleaching while maintaining high image quality. To select small sets of wavelengths we directly searched for the combinations of wavelengths providing the best and worst image quality in comparison with a reference image obtained using 131 closely spaced wavelengths between 680 and 940 nm in terms of the peak signal-to-noise ratio (PSNR). We have shown that using the PSNR optimization method, additional improvements could be achieved over the wavelength set selected using the method of the minimization of the extinction matrix condition number.

多光谱光声层析成像使用可调谐激光波长集提供组织发色团分布的映射。以研究小鼠体内脑脊液动力学的总体目标为基础，我们的工作旨在最大程度地减少波长数量，以减少扫描时间，提高时间分辨率，减少能量沉积并避免示踪剂光漂白，同时保持高图像质量。为了选择小的波长组，我们直接搜索与使用131个在680和940 nm之间的峰值波长信噪比（在680 nm和940 nm之间的紧密间隔的波长）获得的参考图像相比，提供最佳和最差图像质量的波长组合（ PSNR）。我们已经证明，使用PSNR优化方法，