Many industrial activities produce H₂S, which is toxic at high levels and odorous at even very low levels. Chemolithotrophic sulfur-oxidizing bacteria are often used in its remediation. Recently, we have reported that many heterotrophic bacteria can use sulfide:quinone oxidoreductase and persulfide dioxygenase to oxidize H₂S to thiosulfate and sulfite. These bacteria may also potentially be used in H₂S biotreatment. Here we report how various heterotrophic bacteria with these enzymes were cultured with organic compounds and the cells were able to rapidly oxidize H₂S to zero-valence sulfur and thiosulfate, causing no apparent acidification. Some also converted the produced thiosulfate to tetrathionate. The rates of sulfide oxidation by some of the tested bacteria in suspension, ranging from 8 to 50 µmol min⁻¹ g⁻¹ of cell dry weight at pH 7.4, sufficient for H₂S biotreatment. The immobilized bacteria removed H₂S as efficiently as the bacteria in suspension, and the inclusion of Fe₃O₄ nanoparticles during immobilization resulted in increased efficiency for sulfide removal, in part due to chemical oxidation H₂S by Fe₃O₄. Thus, heterotrophic bacteria may be used for H₂S biotreatment under aerobic conditions.

中文翻译：

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硫化物氧化异养细菌对H2S的生物处理。

许多工业活动会产生H ₂ S，该H ₂ S在高水平时有毒，在非常低的水平时有气味。化学营养型硫氧化细菌通常用于其修复。最近，我们报道了许多异养细菌可以使用硫化物：醌氧化还原酶和过硫化物双加氧酶将H ₂ S氧化为硫代硫酸盐和亚硫酸盐。这些细菌也可能用于H ₂ S生物处理。在这里，我们报告了如何用有机化合物培养具有这些酶的各种异养细菌，并使细胞能够快速氧化H _2。S为零价硫和硫代硫酸盐，无明显酸化。一些人还将产生的硫代硫酸盐转化为四硫代酸盐。悬浮液中某些被测细菌的硫化物氧化速率，在pH 7.4时，细胞干重为8至50 µmol min ^-1  g ^-1，足以进行H ₂ S生物处理。固定化细菌与悬浮液中的细菌一样有效地去除了H ₂ S，固定化过程中包含Fe ₃ O ₄纳米颗粒导致硫化物去除效率提高，部分原因是由于Fe ₃ O _4对H ₂ S的化学氧化作用。因此，异养细菌可用于H在有氧条件下进行₂ S生物处理。