Denitrifying granular sludge reactor holds better nitrogen removal efficiency than other kinds of denitrifying reactors, while this reactor commonly needs seeding anaerobic granular sludge and longer period for start-up in practice, which restricted the application of denitrifying granular sludge reactor. This study presented a rapid and stable start-up method for denitrifying granular sludge. An upflow sludge blanket (USB) reactor with packings was established with flocculent activated sludge for treatment of high concentration nitrite wastewater. Results showed mature denitrifying granular sludge appeared only after 15 days with highest nitrogen removal rate of 5.844 kg N/(m³ day), which was much higher than that of compared anoxic sequencing batch reactor (ASBR). No significant nitrite inhibition occurred in USB and denitrification performance was mainly influenced by hydraulic retention time, influent C/N ratio and internal reflux ratio. Hydraulic shear force created by upflow fluid, shearing of gaseous products and stable microorganisms adhesion on the packings might be the reasons for rapid achievement of granular sludge. Compared to inoculated sludge and ASBR, remarkable microbial communitiy variations were detected in USB. The dominance of Proteobacteria and Bacteroidetes and enrichment of species Pseudomonas_stutzeri should be responsible for the excellent denitrification performance, which further verified the feasibility of start-up method.

中文翻译：

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在处理高浓度亚硝酸盐废水的上流污泥层（USB）反应器中，快速启动和反硝化颗粒污泥的性能。

脱氮颗粒污泥反应器比其他类型的脱氮反应器具有更好的脱氮效率，而该反应器通常需要接种厌氧颗粒污泥，而且在实践中需要较长的启动时间，这限制了脱氮颗粒污泥反应器的应用。这项研究提出了一种快速稳定的反硝化颗粒污泥启动方法。用絮凝活性污泥建立了带有填料的上流污泥层反应器，用于处理高浓度的亚硝酸盐废水。结果表明，仅在15天后才出现成熟的反硝化颗粒污泥，脱氮率最高为5.844 kg N /（m ³ 天），远远高于比较的缺氧测序间歇反应器（ASBR）。USB中没有明显的亚硝酸盐抑制作用，反硝化性能主要受水力停留时间，进水C / N比和内部回流比的影响。由上流流体产生的水力剪切力，气态产物的剪切以及稳定的微生物附着在填料上可能是迅速获得颗粒污泥的原因。与接种的污泥和ASBR相比，USB中检测到显着的微生物群落变化。变形杆菌和拟杆菌的优势和丰富的假单胞菌 应该负责出色的脱硝性能，这进一步验证了启动方法的可行性。