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Screening of a beta-cypermethrin-degrading bacterial strain Brevibacillus parabrevis BCP-09 and its biochemical degradation pathway.
Biodegradation ( IF 3.1 ) Pub Date : 2018-08-16 , DOI: 10.1007/s10532-018-9850-0
Jie Tang 1 , Bo Liu 1 , Ting-Ting Chen 1 , Kai Yao 2 , Lin Zeng 1 , Chao-Yi Zeng 1 , Qing Zhang 1
Affiliation  

A novel beta-cypermethrin (Beta-CP)-degrading strain isolated from activated sludge was identified as Brevibacillus parabrevis BCP-09 based on its morphological and physio-biochemical characteristics, and 16S rRNA gene analysis. Strain BCP-09 could effectively degrade Beta-CP at pH 5.0–9.0, 20–40 °C, and 10–500 mg L−1 Beta-CP. Under optimal conditions (pH 7.41, 38.9 °C, 30.9 mg L−1 Beta-CP), 75.87% Beta-CP was degraded within 3 days. Beta-CP degradation (half-life, 33.45 h) and strain BCP-09 growth were respectively described using first-order-kinetic and logistic-kinetic models. Seven metabolites were detected by high-performance liquid chromatography and gas chromatography-mass spectrometry- methyl salicylate, catechol, phthalic acid, salicylic acid, 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid, 3-phenoxybenzaldehyde, and 3-phenoxybenzoic acid (3-PBA). The major Beta-CP metabolite, 3-PBA was further degraded into phenol, benzoic acid, and 4-methylhexanoic acid. BCP-09 also degraded aromatic compounds such as phenol, catechol, and protocatechuic acid. Beta-CP appears to be mainly degraded into 3-PBA, which is continuously degraded into smaller benzene or chain compounds. Thus, strain BCP-09 could form a complete degradation system for Beta-CP and might be considered a promising strain for application in the bioremediation of environments and agricultural products polluted by Beta-CP.

中文翻译:

降解β-氯氰菊酯的细菌菌株副短杆菌BCP-09及其生化降解途径的筛选。

基于活性污泥的形态和生理生化特性以及16S rRNA基因分析,从活性污泥中分离出的新型降解β-氯氰菊酯的菌株被鉴定为副短杆菌BCP-09。BCP-09菌株可以在pH 5.0–9.0、20–40°C和10–500 mg L -1 Beta-CP的条件下有效降解Beta-CP。在最佳条件下(pH 7.41,38.9°C,30.9 mg L -1Beta-CP),75.87%Beta-CP在3天内降解。使用一阶动力学模型和对数动力学模型分别描述了β-CP降解(半衰期为33.45小时)和BCP-09菌株的生长。通过高效液相色谱和气相色谱-质谱法检测到7种代谢物-水杨酸甲酯,邻苯二酚,邻苯二甲酸,水杨酸,3-(2,2-二氯乙烯基)-2,2-二甲基环丙烷甲酸,3-苯氧基苯甲醛和3-苯氧基苯甲酸(3-PBA)。主要的β-CP代谢物3-PBA进一步降解为苯酚,苯甲酸和4-甲基己酸。BCP-09还可以降解芳香族化合物,例如苯酚,儿茶酚和原儿茶酸。Beta-CP似乎主要降解为3-PBA,然后连续降解为较小的苯或链状化合物。从而,
更新日期:2018-08-16
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