A novel beta-cypermethrin (Beta-CP)-degrading strain isolated from activated sludge was identified as Brevibacillus parabrevis BCP-09 based on its morphological and physio-biochemical characteristics, and 16S rRNA gene analysis. Strain BCP-09 could effectively degrade Beta-CP at pH 5.0–9.0, 20–40 °C, and 10–500 mg L⁻¹ Beta-CP. Under optimal conditions (pH 7.41, 38.9 °C, 30.9 mg L⁻¹ Beta-CP), 75.87% Beta-CP was degraded within 3 days. Beta-CP degradation (half-life, 33.45 h) and strain BCP-09 growth were respectively described using first-order-kinetic and logistic-kinetic models. Seven metabolites were detected by high-performance liquid chromatography and gas chromatography-mass spectrometry- methyl salicylate, catechol, phthalic acid, salicylic acid, 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid, 3-phenoxybenzaldehyde, and 3-phenoxybenzoic acid (3-PBA). The major Beta-CP metabolite, 3-PBA was further degraded into phenol, benzoic acid, and 4-methylhexanoic acid. BCP-09 also degraded aromatic compounds such as phenol, catechol, and protocatechuic acid. Beta-CP appears to be mainly degraded into 3-PBA, which is continuously degraded into smaller benzene or chain compounds. Thus, strain BCP-09 could form a complete degradation system for Beta-CP and might be considered a promising strain for application in the bioremediation of environments and agricultural products polluted by Beta-CP.

中文翻译：

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降解β-氯氰菊酯的细菌菌株副短杆菌BCP-09及其生化降解途径的筛选。

基于活性污泥的形态和生理生化特性以及16S rRNA基因分析，从活性污泥中分离出的新型降解β-氯氰菊酯的菌株被鉴定为副短杆菌BCP-09。BCP-09菌株可以在pH 5.0–9.0、20–40°C和10–500 mg L ^-1 Beta-CP的条件下有效降解Beta-CP。在最佳条件下（pH 7.41，38.9°C，30.9 mg L ^-1Beta-CP），75.87％Beta-CP在3天内降解。使用一阶动力学模型和对数动力学模型分别描述了β-CP降解（半衰期为33.45小时）和BCP-09菌株的生长。通过高效液相色谱和气相色谱-质谱法检测到7种代谢物-水杨酸甲酯，邻苯二酚，邻苯二甲酸，水杨酸，3-（2,2-二氯乙烯基）-2,2-二甲基环丙烷甲酸，3-苯氧基苯甲醛和3-苯氧基苯甲酸（3-PBA）。主要的β-CP代谢物3-PBA进一步降解为苯酚，苯甲酸和4-甲基己酸。BCP-09还可以降解芳香族化合物，例如苯酚，儿茶酚和原儿茶酸。Beta-CP似乎主要降解为3-PBA，然后连续降解为较小的苯或链状化合物。从而，