The goal of these studies was to use a tumor-targeting, near-infrared (NIR) fluorescent peptide to evaluate early detection and to guide surgical removal of polyps in a genetically engineered rat model of spontaneous colorectal cancer. This peptide, LS301, was conjugated to Cy7.5 and applied topically to the colon of adenoma-bearing Pirc rats. Ten minutes after administration, rats underwent targeted NIR laser colonoscopy. Rats were also evaluated by white light colonoscopy and narrow-band imaging, for comparison to the NIR technique. Unlike white light and narrow-band colonoscopy, NIR imaging detected unexpected flat lesions in young Pirc rats. NIR imaging was also used to assess resection margins after electrocauterization of polyps. Tumor margins remained negative at 5 weeks postsurgery, demonstrating successful polypectomy. The present studies show that NIR-targeted colonoscopy is an attractive strategy to improve screening for and resection of colorectal neoplasia.

中文翻译：

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评估肿瘤靶向的近红外荧光肽在大肠癌大鼠模型中对息肉的早期发现和内镜切除的效果。

这些研究的目的是使用靶向肿瘤的近红外（NIR）荧光肽评估早期发现并指导在遗传改造的自发性大肠癌大鼠模型中息肉的手术切除。该肽LS301与Cy7.5缀合，并局部施用于带有腺瘤的Pirc大鼠的结肠。给药十分钟后，对大鼠进行靶向NIR激光结肠镜检查。还通过白光结肠镜检查和窄带成像对大鼠进行了评估，以与NIR技术进行比较。与白光和窄带结肠镜检查不同，NIR成像在年轻的Pirc大鼠中检测到意外的扁平病变。NIR成像还用于评估息肉电灼后的切除切缘。术后5周肿瘤切缘仍为阴性，表明息肉切除术成功。