Sex differences in social behaviors exist in mammals during adulthood, and further evidence suggests that sex differences in behavior are present before sexual maturity. In order to model behavioral disorders in animals, it is important to assess baseline sex-related behavioral differences, especially when studying disorders for which sex-related behavioral effects are expected. We investigated the effect of sex on behavior in three strains of prepubertal mice (C57BL/6, CFW, and CF1) using a wheel-running assay. We found no significant sex differences in latency to run on the wheel or total duration of wheel running within each strain. During the social interaction test, there were no differences between the sexes in latency or total duration of contact or following between a subject and a novel mouse. We also evaluated behavioral patterns of wheel running and stereotypical behaviors such as burrowing and grooming. Both sexes showed characteristic wheel-running behavior, spending the majority of each trial interacting with the wheel when it was free and more time performing other activities (e.g., stereotypical behaviors, general locomotion) when it was jammed. These results provide evidence that, among various strains of prepubertal mice, baseline sex-related behavioral differences are not strong enough to influence the measured behaviors.

中文翻译：

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三种小鼠轮跑和社会行为的青春期前性别差异调查

成年哺乳动物的社会行为存在性别差异，进一步的证据表明，行为的性别差异在性成熟之前就存在。为了模拟动物的行为障碍，评估基线与性别相关的行为差异很重要，尤其是在研究预期与性别相关的行为影响的障碍时。我们使用轮跑试验研究了性别对三种青春期前小鼠品系（C57BL/6、CFW 和 CF1）行为的影响。我们发现在车轮上运行的潜伏期或每个菌株内车轮运行的总持续时间没有显着的性别差异。在社交互动测试期间，受试者与新型小鼠之间的潜伏期或接触或跟随的总持续时间在性别之间没有差异。我们还评估了车轮运行的行为模式和刻板行为，例如挖洞和梳理毛发。两性都表现出典型的车轮运行行为，每次试验的大部分时间都在轮子空闲时与轮子互动，而在轮子被卡住时花更多时间进行其他活动（例如，刻板行为、一般运动）。这些结果提供的证据表明，在不同品系的青春期前小鼠中，与性别相关的基线行为差异不足以影响所测量的行为。一般运动）当它被卡住时。这些结果提供的证据表明，在不同品系的青春期前小鼠中，与性别相关的基线行为差异不足以影响所测量的行为。一般运动）当它被卡住时。这些结果提供的证据表明，在不同品系的青春期前小鼠中，与性别相关的基线行为差异不足以影响所测量的行为。