Twenty clinical Pseudomonas aeruginosa isolates recovered from patients admitted to The General Hospital in Ismailia Governorate (Egypt) were examined in this study. We analysed P. aeruginosa ATCC 9027 (as a control strain) and 19 of the isolates after digestion with SpeI restriction endonuclease. After this we conducted a pulsed-field gel electrophoresis (PFGE) and typed the obtained 10 unique patterns, designated as A, A1, B, B1, C, C1, D, D1, E and F. We evaluated the genetic relatedness between all strains, based on ≥87% band identity. As a result, the isolates were grouped in the 10 clusters as follows: patterns A, A1, B, B1, C contained two strains each and patterns C1, D, D1, E contained a single strain each; the five remaining strains were closely related (genomic pattern F). One isolate belonged to antibiotype ‘b’. The genotype patterns of the P. aeruginosa ATCC 9027 control strain and isolate no. 11 were closely related and had two different antibiotypes ‘d’ and ‘c’, respectively.

中文翻译：

---

脉冲场凝胶电泳快速鉴定铜绿假单胞菌

本研究检查了从伊斯梅利亚省（埃及）总医院收治的患者中回收的 20 株临床铜绿假单胞菌分离株。我们分析了铜绿假单胞菌 ATCC 9027（作为对照菌株）和用 SpeI 限制性核酸内切酶消化后的 19 个分离株。在此之后，我们进行了脉冲场凝胶电泳 (PFGE) 并对获得的 10 个独特的模式进行了打字，命名为 A、A1、B、B1、C、C1、D、D1、E 和 F。我们评估了所有这些模式之间的遗传相关性。菌株，基于≥87% 的条带同一性。结果，分离物被分为 10 个簇，如下所示：模式 A、A1、B、B1、C 各包含两个菌株，模式 C1、D、D1、E 各包含一个菌株；剩下的五个菌株密切相关（基因组模式 F）。一种分离株属于抗生素“b”型。铜绿假单胞菌 ATCC 9027 对照菌株和分离株的基因型模式。11 种密切相关，分别具有两种不同的抗生素型“d”和“c”。