AIM To study the antioxidant action of five polyphenols (+) catechin, procyanidin B2, procyanidin B5, procyanidin B5 3'-O-gallate, and amurensisin isolated from the seeds of Vitis amurensis. METHODS The mouse liver homogenate lipid peroxidation assay was applied for the evaluation of the antioxidant activity in vitro. RESULTS (+) Catechin, procyanidin B2, procyanidin B5, procyanidin B5 3'-O-gallate, and amurensisin showed antioxidant activity with the IC50 values of 0.47, 0.25, 0.10, 0.02, and 0.03 mmol/L, respectively. The IC50 value of vitamin E used as a positive control was 0.13 mmol/L. The structural activity relationship was also analyzed. Procyanidins carrying a galloyl group possessed higher anti-lipid peroxidation activities. All dimers were found to be more potent than the non-galloylated momomer such as (+) catechin. However, the activity of the 4-->6 linked dimer seemed more preferable than 4-->8 linked dimer. CONCLUSION Procyanidin B5, procyanidin B5 3'-O-gallate, and amurensisin showed a more antioxidant activity than vitamin E did, and their activity is dependent on their substitution and polymerization patterns.

中文翻译：

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紫葡萄种子中多酚的体外抗氧化活性

目的研究从葡萄籽中分离得到的5种多酚（+）儿茶素，原花青素B2，原花青素B5，原花青素B5 3'-O-没食子酸酯和阿穆那霉素的抗氧化作用。方法采用小鼠肝匀浆脂质过氧化法评价其体外抗氧化活性。结果（+）儿茶素，原花青素B2，原花青素B5，原花青素B5 3'-O-没食子酸酯和阿穆里斯丁显示出抗氧化活性，IC50值分别为0.47、0.25、0.10、0.02和0.03 mmol / L。用作阳性对照的维生素E的IC50值为0.13 mmol / L。还分析了结构活性关系。带有没食子酰基的原花青素具有较高的抗脂质过氧化活性。发现所有二聚体均比未加铝基化的单体（如（+）儿茶素）更有效。然而，4-> 6连接的二聚体的活性似乎比4-> 8连接的二聚体的活性更好。结论原花青素B5，原花青素B5 3'-O-没食子酸酯和阿穆那丁显示出比维生素E更高的抗氧化活性，它们的活性取决于它们的取代和聚合方式。