Phylogenetic analyses of four nuclear genes, namely the large and small subunits of the nuclear ribosomal RNA, transcription elongation factor 1-alpha and the second largest RNA polymerase II subunit, established that the ecological group of marine bitunicate ascomycetes has representatives in the orders Capnodiales, Hysteriales, Jahnulales, Mytilinidiales, Patellariales and Pleosporales. Most of the fungi sequenced were intertidal mangrove taxa and belong to members of 12 families in the Pleosporales: Aigialaceae, Didymellaceae, Leptosphaeriaceae, Lenthitheciaceae, Lophiostomataceae, Massarinaceae, Montagnulaceae, Morosphaeriaceae, Phaeosphaeriaceae, Pleosporaceae, Testudinaceae and Trematosphaeriaceae. Two new families are described: Aigialaceae and Morosphaeriaceae, and three new genera proposed: Halomassarina, Morosphaeria and Rimora. Few marine species are reported from the Dothideomycetidae (e.g. Mycosphaerellaceae, Capnodiales), a group poorly studied at the molecular level. New marine lineages include the Testudinaceae and Manglicola guatemalensis in the Jahnulales. Significantly, most marine Dothideomycetes are intertidal tropical species with only a few from temperate regions on salt marsh plants (Spartina species and Juncus roemerianus), and rarely totally submerged (e.g. Halotthia posidoniae and Pontoporeia biturbinata on the seagrasses Posidonia oceanica and Cymodocea nodosum). Specific attention is given to the adaptation of the Dothideomycetes to the marine milieu, new lineages of marine fungi and their host specificity.

Taxonomic novelties: Aigialaceae Suetrong, Sakayaroj, E.B.G. Jones, Kohlm., Volkm.-Kohlm. & Schoch, fam. nov., Halomassarina Suetrong, Sakayaroj, E.B.G. Jones, Kohlm., Volkm.-Kohlm. & Schoch, gen. nov., Halomassarina thalassiae (Kohlm. & Volkm.-Kohlm.), Suetrong, Sakayaroj, E.B.G. Jones, Kohlm., Volkm.-Kohlm. & Schoch, comb. nov., Suetrong, Sakayaroj, E.B.G. Jones, Kohlm., Volkm.-Kohlm., comb. nov., Clade V. Morosphaeriaceae Suetrong, Sakayaroj, E.B.G. Jones, & Schoch, fam. nov., Morosphaeria velataspora (K.D. Hyde & Borse) Suetrong,· Sakayaroj, E.B.G. Jones & Schoch, comb. nov., Morosphaeria ramunculicola (K.D. Hyde) Suetrong,· Sakayaroj, E.B.G. Jones & Schoch, comb. nov., Rimora Kohlm., Volkm-Kohlm., Suetrong, Sakayaroj, E.B.G. Jones, gen. nov., Rimora mangrovei (Kohlm. & Vittal) Kohlm., Volkm-Kohlm., Suetrong, Sakayaroj, E.B.G. Jones, comb. nov.

对四个核基因（即核核糖体RNA的大小亚基、转录延伸因子1-α和第二大RNA聚合酶II亚基）的系统发育分析表明，海洋双囊子囊菌的生态群在Capnodiales目中具有代表， Hysteriales 、 Jahnulales 、 Mytilinidiales 、 Patellariales和Pleosporales 。测序的大部分真菌为潮间带红树林类群，属于Pleosporale s 的 12 个科的成员： Aigialaceae 、 Didymellaceae 、 Leptosphaeriaceae 、 Lenthitheciaceae 、 Lophiostomataceae 、 Massarinaceae 、 Montagnulaceae 、 Morosphaeriaceae 、 Phaeosphaeriaceae 、 Pleosporaceae 、 Testudinaceae和槐科。描述了两个新科： Aigialaceae和Morosphaeriaceae ，并提出了三个新属： Halomassarina 、 Morosphaeria和Rimora。很少有来自Dothideomycetidae 的海洋物种报道（例如Mycosphaerellaceae 、 Capnodiales ），该类群在分子水平上的研究很少。新的海洋谱系包括Jahnulales中的Testudinaceae和Manglicola guatemalensis 。值得注意的是，大多数海洋Dothideomycetes是潮间带热带物种，只有少数来自温带地区的盐沼植物（大米草属和灯心草），并且很少完全淹没（例如 海草Posidonia oceanica和Cymodocea nodosum上的Halotthia posidoniae和Pontoporeia biturbinata 。特别关注Dothideomycetes对海洋环境的适应、海洋真菌的新谱系及其宿主特异性。

分类学新颖性： Aigialaceae Suetrong、Sakayaroj、EBG Jones、Kohlm.、Volkm.-Kohlm。 & 肖赫，家人。十一月， Halomassarina Suetrong，Sakayaroj，EBG Jones，Kohlm.，Volkm.-Kohlm。 & 肖赫，将军。十一月， Halomassarina thalassiae (Kohlm. & Volkm.-Kohlm.)，Suetrong，Sakayaroj，EBG Jones，Kohlm.，Volkm.-Kohlm。 & Schoch，梳子。十一月，Suetrong，Sakayaroj，EBG Jones，Kohlm.，Volkm.-Kohlm.，梳子。 11 月，Clade V. Morosphaeriaceae Suetrong，Sakayaroj，EBG Jones 和 Schoch，fam。 11 月， Morosphaeria velataspora (KD Hyde & Borse) Suetrong，Sakayaroj，EBG Jones & Schoch，梳子。 11 月， Morosphaeria ramunculicola (KD Hyde) Suetrong，Sakayaroj，EBG Jones & Schoch，梳子。十一月， Rimora Kohlm.，Volkm-Kohlm.，Suetrong，Sakayaroj，EBG Jones，gen。十一月， Rimora mangrovei (Kohlm. & Vittal) Kohlm.，Volkm-Kohlm.，Suetrong，Sakayaroj，EBG Jones，梳子。十一月