The objective of this study was to investigate the electrical impedance properties of rat lung and other tissues ex vivo using Electrical Impedance Spectroscopy. Rat lungs (both electroporated and naïve (untreated)), and mesenteric vessels (naïve) were harvested from male Sprague-Dawley rats; their electrical impedance were measured using a Solartron 1290 impedance analyzer. Mouse lung and heart samples (naïve) were also studied. The resistance (Real Z, ohm) and the reactance (Im Z, negative ohm)) magnitudes and hence the Cole-Cole (Real Z versus Im Z) plots are different for the electroporated lung and the naive lung. The results confirm the close relationship between the structure and the functional characteristic. These also vary for the different biological tissues studied. The impedance values were higher at low frequencies compared to those at high frequencies. This study is of practical interest for biological applications of electrical pulses, such as electroporation, whose efficacy depends on cell type and its electrical impedance characteristics.

中文翻译：

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生物组织的电阻抗谱研究

本研究的目的是使用电阻抗光谱法研究离体大鼠肺和其他组织的电阻抗特性。从雄性 Sprague-Dawley 大鼠中采集大鼠肺（电穿孔和幼稚（未经处理））和肠系膜血管（幼稚）；使用 Solartron 1290 阻抗分析仪测量它们的电阻抗。还研究了小鼠肺和心脏样本（未处理）。电阻 (Real Z, ohm) 和电抗 (Im Z,negative ohm)) 大小以及 Cole-Cole（Real Z 与 Im Z）图对于电穿孔肺和幼稚肺是不同的。结果证实了结构与功能特性之间的密切关系。这些也因所研究的不同生物组织而异。与高频时相比，低频时的阻抗值更高。这项研究对电脉冲的生物学应用具有实际意义，例如电穿孔，其功效取决于细胞类型及其电阻抗特性。